Buta-1,3-diene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Non-GLP, non-guideline study, published in peer reviewed literature, restrictions in design and/or reporting.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

publication

Title:

Concentrations of hydrocarbons in tissues as a measure of toxicity,

Author:

Shugaev B

Year:

1969

Bibliographic source:

Arch. Environ. Health, 18, 878-882.

Materials and methods

Principles of method if other than guideline:

Method: other

GLP compliance:

no

Remarks:

predates GLP

Test type:

other: LC50s but no method details

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Buta-1,3-diene

EC Number:

203-450-8

EC Name:

Buta-1,3-diene

Cas Number:

106-99-0

Molecular formula:

C4H6

IUPAC Name:

buta-1,3-diene

Constituent 2

Reference substance name:

1,3-Butadiene

Cas Number:

106-99-0

Molecular formula:

C4H6

IUPAC Name:

1,3-Butadiene

Details on test material:

1,3-Butadiene (source and purity not specified)

Test animals

Species:

other: rat and mouse

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

not given

Administration / exposure

Route of administration:

inhalation: gas

Type of inhalation exposure:

not specified

Vehicle:

other: air

Details on inhalation exposure:

Described as "dynamic flow exposure"

Analytical verification of test atmosphere concentrations:

no

Duration of exposure:

>= 2 h

Remarks on duration:

Rats were exposed for 4h mice were exposed for 2h

Concentrations:

Specific concentrations not given

No. of animals per sex per dose:

Not specified

Control animals:

not specified

Details on study design:

see below

Statistics:

see below

Results and discussion

Preliminary study:

Not applicable

Effect levels

Effect levels 1

Key result

Sex:

not specified

Dose descriptor:

LC50

Effect level:

285 other: mg/L air (285, 000 mg/m3)

Exp. duration:

4 h

Remarks on result:

other: rat

Effect levels 2

Key result

Sex:

not specified

Dose descriptor:

LC50

Effect level:

270 other: mg/L (270,000 mg/m3)

Exp. duration:

2 h

Remarks on result:

other: mice

Mortality:

None

Clinical signs:

other:

Body weight:

Not determined

Gross pathology:

Not determined

Other findings:

none

Any other information on results incl. tables

Rat LC50 (4 hr) = 285 mg/L; confidence limits 219 -370 mg/L (p<0.05).
Mouse LC50 (2 hr) = 270 mg/L; confidence limits 251-290 mg/L (p<0.05).

Toxic effects are described in Shugaev, B.B. (1968), Farmakol. Toksikol. (Moscow) 31,162-165. There was a rapid onset of narcosis. This was reversible, after 30min withdrawal from treatment all animals returned to normal. At lethal concentrations breathing become shallower, rarer and periodic.  

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Criteria used for interpretation of results: EU

Conclusions:

This is an old non-guideline study that is not up to current standards, but there are few acute toxicity studies available on 1,3-butadiene. However LC50s were reported to be 285 mg/L for 4h (285,000 mg/m3) in rats, 270 mg/L for 2h (270,000mg/m3) in mice.

Executive summary:

Rats and mice were exposed to 1,3-butadiene for 4h or 2h respectively. There was a rapid but reversible onset of narcosis. LC50 values were reported to be 285 mg/L (285,000 mg/m³) in rats and 270 mg/L (270,000 mg/m³) in mice.