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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
EPA guideline similar to OECD-guideline.
Qualifier:
according to guideline
Guideline:
EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
GLP compliance:
yes
Specific details on test material used for the study:
purity: 99.72 %
Lot 5-0523-88 was received frozen from Rohm and Haas Company and stored at room temperature. The substance was a clear light yellow liquid.
Analytical monitoring:
yes
Details on sampling:
At each sampling period 5 ml aliquots of test water were collected from each of the four replicates of each test solution. Two replicates at each time
point were composited.
Vehicle:
no
Details on test solutions:
A primary stock standard of MAA was prepared and stored at room temperature. Subsequent dilutions were prepared in Milipore water for use as
spiking solutions and test dilution water.
Test organisms (species):
Daphnia magna
Details on test organisms:
ABC Laboratories in house culture. The primary culture was obtained from the Columbia National Fisheries Research Laboratory (CNFRL), Columbia, Missouri, in 1977
Age at study initiation: first instar (<=24 hours old).
Organisms were held in temperature controlled area at 20°C.
Lighting was 50-70 footcandles on a 16 hr photoperiod, with 30 minute dawn and dusk transition periods.
During the holding period, the daphnids were fed a suspension of algae (Selenastrum capricornutum) supplemented with Tetramin®/cereal leaves/yeast suspension.
Organisms were not fed during the 48 hr treatment period.
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
172-178 mg/L
Test temperature:
20°C
pH:
7.0-7.9
Dissolved oxygen:
7.7-8.2 mg/L
Conductivity:
310-365 μMH0S/cm
Nominal and measured concentrations:
Nominal = 7.2, 14, 30, 60 and 120 mg/L.
Mean measured (sampling at 0 and 48 hrs) = 6.7, 13, 32, 62 and 130 mg/L.
Details on test conditions:
Method: flow-through bioassay as described in American Public Health Association and Standard Methods for the Examination of Water and 
Wastewater.

A half liter proportional diluter system was used for intermittent introduction of dilution water and MAA into the test chambers. The test system contained six sets of four replicate one liter test chambers designated as control, and level 1 through level 5. The proportional diluter was calibrated preior to use in the test.

Dilution water was a blend of reverse osmosis water and ABC laboratory well water.

10 organisms were assigned to each of the four replicate test chambers (40 Daphnids per concentration).
Water quality measurements were recorded at 0 and 48 hours.

Additional chemical Characteristics of Hard Blended Watera
Total 0rganic Carbon: <1 .O pprn
Suspended Bolids: 0.5 ppm
Un-ionized Arnmonia: <0.00262 ppm
Chlorine (TRC): <0.05 ppm
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
130 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: based on lack of immobilization and or abnormal effects
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 130 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: The 48 hr dose response slope could not be determined because of the lack of an effect level
Details on results:
Overall, the measured concentrations represent 101-7.4% of nominal concentrations. Quality control samples analyzed with each sample day over a range of 6.00 to 150 mg/L with recoveries averaging 94+/-19%. Low recovery for the 0 hour sampling was obtained. Duplicate low spikes at teh 48 hour sampling were analyzed to verify the recovery of MAA at the 6.00 mg/l concentration.

Measurement at 0 and 48 hrs of the MAA diluter stock solution averaged 12,000 mg/L which represented 100% of the nominal concentration (12,000 mg/L).
Validity criteria fulfilled:
yes
Conclusions:
In a valid guideline study, the 48 hour EC50 for Daphnia magna was >130 mg/L MAA.
Executive summary:

Methacrylic acid was tested in an acute test to aquatic invertebrates acc. EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity test Test) with Daphnia magna. The definitive flow-through acute toxicity of Methacrylic Acid to Daphnia magna was conducted the following nominal concentrations: 7.2, 14, 30, 60 and 120 mg/1.

Forty first-instar daphnids were exposed to each of the test concentrations and control.

The mean measured concentrations calculated from analytical sampling at 0 and 48 hours are as follows: 6.7, 13, 32, 62 and 130 mg/1. The toxicity of Methacrylic Acid based on mean measured test

concentrations were EC50 (48h): > 130 mg/l, NOEC (48h): 130 mg/l

Water quality parameters of temperature, dissolved oxygen and pH were measured in all test concentrations at 0- and 48-hours and were determined to be within acceptable limits. The overall ranges for these

parameters were: daily diluter temperature observations = 20°C, pH = 7.0 - 7.9 and dissolved oxygen= 7.7 - 8.2 mg/1.

Description of key information

Methacrylic acid was tested in an acute test to aquatic invertebrates acc. EPA OTS 797.1300 (Aquatic Invertebrate Acute Toxicity Test) with Daphnia magna. The definitive flow-through acute toxicity of Methacrylic Acid to Daphnia magna was conducted the following nominal concentrations: 7.2, 14, 30, 60 and 120 mg/1.


Forty first-instar daphnids were exposed to each of the test concentrations and control.


The mean measured concentrations calculated from analytical sampling at 0 and 48 hours are as follows: 6.7, 13, 32, 62 and 130 mg/1. The toxicity of Methacrylic Acid based on mean measured test


concentrations were EC50 (48h): > 130 mg/l, NOEC (48h): 130 mg/l


Water quality parameters of temperature, dissolved oxygen and pH were measured in all test concentrations at 0- and 48-hours and were determined to be within acceptable limits. The overall ranges for these


parameters were: daily diluter temperature observations = 20°C, pH = 7.0 - 7.9 and dissolved oxygen= 7.7 - 8.2 mg/1.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Remarks:
48 h
Effect concentration:
130 mg/L

Additional information

In an additional acute toxicity test acc. OECD 202 (Degussa 1990) methacrylic acid was tested at nominal concentrations of 10, 18, 32, 56, 100, 180 and 320 mg/l. pH at the highest test concentrations was lower (pH 4.8 and 4.2) in comparison to the lower test concentrations. EC50 (24 h) was > 100 mg/, NOEC (24h) was 56 mg/l based on immobilization.

The marine ecotoxicity of Methacrylic acid was tested in the marine invertebrate Acartia tonsa (Copepoda) in natural seawater for 48 hours. The 48 h LC50 in the marine invertebrate Acartia tonsa (Copepoda) was 210 mg/L.

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