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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Although the study has been conducted with triiron tetraoxide at a test concentration well exceeding the solubility the test is deemed appropriate as supporting evidence for the non-classification of triiron tetraoxide products

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Principles of method if other than guideline:
Directive 92/69/EEC, C.1, equivalent to the OECD 203
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
1317-277-5
IUPAC Name:
1317-277-5
Constituent 2
Reference substance name:
Triiron tetraoxide
EC Number:
215-277-5
EC Name:
Triiron tetraoxide
Cas Number:
1317-61-9
Constituent 3
Reference substance name:
iron(II,III) oxide
IUPAC Name:
iron(II,III) oxide
Details on test material:
Bayferrox 350 liquid, purity not specified
Composition:
ca. 50 % Iron oxide black (pigment) in aqueous suspension
1 % dispersing agent
0.25 % biocide
0.03 % thickening agent
rest: water

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LL0
Effect conc.:
> 10 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Considering the heterogeneous composition of the test substance, a Water

Accommodated Fraction (= WAF) of Bayferrox 350 liquid was tested

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
LL0 and LC50 > 10000 mg/L
Executive summary:

For triiron tetraoxide a 96-hour LL0 (lethal loading) of >= 10000 mg/l (nominal concentration) was observed on Brachydanio rerio. Directive 92/69/EEC, C.1, equivalent to the OECD 203 was the method used.