Nickel dinitrate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

not reported

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study without detailed documentation. Lacked information on the purity of the test substance and study was conducted with only a single bacterial strain.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

publication

Title:

Study of the mutagenicity of metal derivatives with Salmonella typhimurium TA102.

Author:

Marzin, D.R. and H.V. Phi

Year:

1985

Bibliographic source:

Mutation Research. 155(1/2):49-51.

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Maron and Ames

Principles of method if other than guideline:

Mutagenicity assay, conducted as described by Maron and Ames (1983) in the Ames plate-incorporation test.  

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Nickel dinitrate

EC Number:

236-068-5

EC Name:

Nickel dinitrate

Cas Number:

13138-45-9

Molecular formula:

Ni(NO3)2

IUPAC Name:

nickel(2+) dinitrate

Constituent 2

Reference substance name:

Merck.

IUPAC Name:

Merck.

Details on test material:

- Name of test material (as cited in study report): Nickel nitrate
- Molecular formula (if other than submission substance): not different than submission substance
- Molecular weight (if other than submission substance): not different than submission substance
- Smiles notation (if other than submission substance): not different than submission substance
- InChl (if other than submission substance): not different than submission substance
- Structural formula attached as image file (if other than submission substance): not different than submission substance
- Substance type: pure product
- Other details not reported or not applicable

Method

Target gene:

not applicable

Species / strain

Species / strain / cell type:

S. typhimurium TA 102

Details on mammalian cell type (if applicable):

not applicable

Additional strain / cell type characteristics:

not specified

Metabolic activation:

without

Test concentrations with justification for top dose:

0, 10, 30, 100, 300, and 1000 nM/plate

Vehicle / solvent:

- Vehicle(s)/solvent(s) used: medium

Controls

Untreated negative controls:

yes

Remarks:

water

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

mitomycin C

Details on test system and experimental conditions:

METHOD OF APPLICATION:
S. typhimurium strain TA102 was obtained from Prof. B.N. Ames, University of California, Berkely.   
Results were generated as a mean of triplicate plates, with parallel negative (water) and positive (mitomycin c) controls.  
Test substance doses were a logarithmic series up to insolubility, or toxicity.  
The threshold toxic dose was determined to be that which decreased the estimated background bacterial lawn under microscopic examination.

OTHER: details not reported

Evaluation criteria:

Criteria for mutagenicity:
- dose/effect relationship observed,
- effect is reproducible, and
- number of revertants = 2 x number of spontaneous revertants.

Statistics:

Not reported

Results and discussion

Test results

Species / strain:

S. typhimurium TA 102

Metabolic activation:

without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Additional information on results:

The test substance was determined not to be mutagenic to S. typhimurium strain TA102.  
The threshold toxic dose was reported to be 3000 nM/plate.

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Nickel nitrate was determined not to be mutagenic to S. typhimurium strain TA102. 

Executive summary:

ROBUST SUMMARY DEVELOPED BY AN INDEPENDENT REVIEWER.

Mutagenicity assay, conducted as described by Maron and Ames (1983) in the Ames plate-incorporation test.  S. typhimurium strain TA102 was  

obtained from Prof. B.N. Ames, University of California, Berkely.  Results were generated as a mean of triplicate plates, with parallel  

negative (water) and positive (mitomycin c) controls.  Test substance doses were a logarithmic series up to insolubility, or toxicity.  The  

threshold toxic dose was determined to be that which decreased the estimated background bacterial lawn under microscopic examination.

Criteria for mutagenicity:
-dose/effect relationship observed,
-effect is reproducible, and
-number of revertants = 2 x number of spontaneous revertants.

The test substance was determined not to be mutagenic to S. typhimurium strain TA102.  The threshold toxic dose was reported to be 3000 nM/plate.

STUDY RATED BY AN INDEPENDENT REVIEWER.