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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Principles of method if other than guideline:
Experiments were performed in aquariums under static conditions. Study comprised of 5-7 concentrations arranged in arithmetical progression and a control group. Each concentration was tested at 8 or 12 fish. 48-hr LC50 values and 95% confidence interval wee determined. Test was performed twice.
GLP compliance:
not specified
Analytical monitoring:
yes
Details on sampling:
- Sampling method: solutions were replaced every 24h. Before and after change of solutions, oxygen contents, temperature and pH of these solutions were measured.
Vehicle:
yes
Details on test solutions:
- Concentrations: range 50 - 140 mg/L (5-7 concentrations tested in arithmetical progression and one control group)
Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: carp
- Age at study initiation (mean and range, SD): 0+
- Weight at study initiation (mean and range, SD): 33.6 +/- 15.5 g
- Feeding during test: no
- Number of fish: 8-12 fish at each concentration

ACCLIMATION
- Acclimation period: 10-14 days
- Acclimation conditions (same as test or not): not indicated
- Type and amount of food: no
- Health during acclimation (any mortality observed): not indicated


Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
not indicated
Hardness:
3.20-3.34 mval
Test temperature:
12.0-15.2 C
pH:
7.2-8.4
Dissolved oxygen:
9.0-9.8 mg/L
Salinity:
14.0-11.0 mg/L Na
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 245 dm3 aquariums
- Aeration: steady
- Renewal rate of test solution (frequency/flow rate): 24 h


TEST MEDIUM / WATER PARAMETERS
- Total organic carbon: not indicated
- Chlorine: 17.8 - 13.2 mg/L
- Fe: 0.16-0.27 mg/L
- SiO2: 8.8-8.2 mg/L
- SO4: 19.0-13.6 mg/L
- HCO3: 352-170 mg/L
- Mg: 18.0-9.3 mg/L
- Mn: 0.0 mg/L
- Ca: 78.0-52.5 mg/L
- K: 6.4-3.3 mg/L
- Conductivity: 362 - 340 uS/cm
Reference substance (positive control):
yes
Remarks:
negative control group
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
447 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
other: calculated based on total ammonia
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 95 - < 102 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
other: total ammonia
Basis for effect:
mortality (fish)
Remarks on result:
other: (83-109 and 78-134)
Validity criteria fulfilled:
yes
Conclusions:
48-h LC50 is 102 mg total ammonia/L equivalent to 480 mg ammonium nitrate/L (95% CI 367-630) when 8 fish/concentration were exposed. 48-h LC50 is 95 mg total ammonia/L equivalent to 447 mg ammonium nitrate/L (95% CI 391-513) when 12 fish/concentration were exposed.
Conclusion: 48-h LC50 for ammonium nitrate is 447 mg/L (95% CI 391-513).

Description of key information

No reliable study with nitric acid, ammonium calcium salt itself is available. The 48-h LC50 for ammonium nitrate in fish (carp) is 447 mg/L (95% CI 391-513). The read-across rationale can be found in the document attached in the target record.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
447 mg/L

Additional information

No reliable study with nitric acid, ammonium calcium salt itself is available. However, ammonium nitrate was tested in cyrpinus carpio, carp, for 48 hours. Based on the total ammonia concentration the LC50 was approx. 100 mg/L, calculating the LC50 for ammonium nitrate to be 447 mg/L. Although an exposure period of only 48 hr was considered here, it is thought that although the 96 hr LC50 will be lower, this will not be lower than 100 mg/L.

In the aquatic environment, all the present nitrate substances (see summary aquatic toxicity) will dissociate into their ions: ammonium, calcium, magnesium, nitrate, potassium and sodium. From the substances tested, it is clear that the LC50 on fish is higher then 100 mg/L.

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