N-cyclohexylbenzothiazole-2-sulfenamide

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: acceptable documented study report, which meets basic scientific principles

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1973

Report date:

1973

Reference 2

Title:

Acute toxicologic evaluation of Santocure vulcanization accelerator

Author:

Randall, D.J. and Bannister, R.M.,

Year:

1990

Bibliographic source:

Acute Toxic. Data 1 (2), 105-106

Materials and methods

Principles of method if other than guideline:

The acute oral toxicity of CBS was evaluated in an acceptable documented study with Sprague-Dawley Albino rats. A 25 % solution-suspension of CBS in corn oil was administered by gavage to 4 groups of 5 Sprague-Dawley albino rats at doses of 3980, 5010, 6310 and 7940 mg/kg bw. A 7-day observation period followed administration.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-cyclohexylbenzothiazole-2-sulfenamide

EC Number:

202-411-2

EC Name:

N-cyclohexylbenzothiazole-2-sulfenamide

Cas Number:

95-33-0

Molecular formula:

C13H16N2S2

IUPAC Name:

N-(1,3-benzothiazol-2-ylsulfanyl)cyclohexanamine

Details on test material:

IUCLID4 Test substance: other TS: as prescribed by chapter 1 in dataset of Monsanto

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Doses:

3980, 5010, 6310, 7940 mg/kg bw

No. of animals per sex per dose:

5 per dose group (mixed males and females; 2-3 males per group, 2-3 females per group).

Control animals:

no

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

5 300 mg/kg bw

Based on:

test mat.

Remarks on result:

other: Signs of Intoxication: Reduced appetite and activity (two to four days in survivors), increased weakness, ocular discharge, slight tremors, collapse, and death Gross autopsy: lung and liver hyperemia and acute gastrointestinal inflammation.

Mortality:

3980 mg/kg bw: 0/5 animals died; 5010 mg/kg bw: 1/5 animals died; 6310 mg/kg bw: 4/5 animals died; 7940 mg/kg bw: 4/5 animals died.

Clinical signs:

other: Reduced appetite and activity (two to four days in survivors), increased weakness, ocular discharge, slight tremors, collapse, and death.

Gross pathology:

Decendents: lung and liver hyperemia and acute gastrointestinal inflammation.
Survivors: Vicera of surviving animals appeared normal at sacrifice.

Any other information on results incl. tables

Signs of Intoxication: Reduced appetite and activity (two to four days in survivors), increased weakness, ocular discharge, slight tremors, collapse, and death.

Gross autopsy:

Decendents: lung and liver hyperemia and acute gastrointestinal inflammation.

Survivors: Vicera of surviving animals appeared normal at sacrifice.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

LD50 = 5300 mg/kg bw.

Executive summary:

The acute oral toxicity of CBS was evaluated in an acceptable documented study with Sprague-Dawley Albino rats. A 25 % solution-suspension of CBS in corn oil was administered by gavage to t groups of 5 (mixed males and females) Sprague-Dawley albino rats at doses of 3980, 5010, 6310 and 7940 mg/kg bw. A 7-day observation period followed administration. The acute oral LD50 for CBS for male and female rats was calculated to be 5300 mg/kg bw (Monsanto Co. 1973).