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Diss Factsheets
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EC number: 200-821-6 | CAS number: 74-90-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study precedes adoption of OECD Guideline. However, the ECETOC task force on Cyanides evaluated the data and found its conclusions to be valid and consistent with other studies.
Data source
Reference
- Reference Type:
- publication
- Title:
- Vergleichende wasser-toxikologische Untersuchungen an Bakterien, Algen und Kleinkrebsen
- Author:
- Bringmann G, Kühn, R
- Year:
- 1 959
- Bibliographic source:
- Gesundheits-Ingenieur 80, 115 - 120
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- no
- Remarks:
- preceeds establishment of GLP protocols
Test material
- Reference substance name:
- Potassium cyanide
- EC Number:
- 205-792-3
- EC Name:
- Potassium cyanide
- Cas Number:
- 151-50-8
- IUPAC Name:
- potassium cyanide
Constituent 1
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- -Source (laboratory, culture, collection): Cultured at testing facility Bundesgesundheitsamt, Institut fur Wasser-, Boden- und Lufthygiene, Berlin-Dahlem, Germany
Method: Cultivation of stock cultures performed in 100 ml of a culture medium consisting of municipal wastewater and a nutrient solution (NO3 570 mg/L, CaSO4 200 mg/L, KH2PO4 140 mg/L, MgSO4 90 mg/L, FeCl3 3 mg/L) at daylight at 24 degrees C in 300 ml Erlenmeyer flasks. One week prior to testing, aliquots of the cultures are transferred into new culture medium and incubated for 1 week at 24 degrees C and illumination (24 hr fluorescent tubes Osram NHI 40 W and Osram HNT 40 W). Algae are filtered into distilled water. The turbidity of the culture is determined.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
Test conditions
- Details on test conditions:
- DETAILS ON TEST CONDITIONS
TEST SYSTEM
-Test vessel: Type: closed glass tubes
-Type: closed
-Material, size, headspace, fill volume: closed galls tubes, diameter 18 mm
-Aeration: No
-Initial cell density: No data
-Control end cell density: No data
GROWTH MEDIUM:
-Detailed composition if non-standard medium was used: filtered waste water (membrane filer 0.6-1.0 micron)
TEST MEDIUM/WATER PARAMETERS:
-Source/preparation of dilution water: River Havel (Gatow, Germany)
-Culture medium different from test medium: Yes
OTHER TEST CONDITIONS
-Sterile test conditions: No
-Adjustment of pH: No
-Photoperiod: 24 h
-Light intensity: Fluorescent tubes Osram HNI 40 W and Osram HNT 40 W
EFFECT PARAMETERS MEASURED (with observation intervals, if applicable):
-Determination of cell concentrations: colorimetric measurements of turbidity of the test cultures after 96 h at end of test.
TEST CONCENTRATIONS:
Results and discussion
Effect concentrations
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- In a 96 hour acute toxicity study, the cultures of Desmodesmus subspicatus (reported as Scenedesmus subspicatus) were exposed to potassium cyanide at nominal concentrations of potassium cyanide (CAS-No. 151-50-8) under static conditions. No guideline was followed, but the aim of the study was to develop test methods to determine whether pollutants affect self-purification of surface waters. The 96h LOEC value based on cell density (turbidity of the test cultures) was 0.16 mg active ingredient./L.
Potassium cyanide (KCN, CAS-No. 151-50-8) is very toxic against the green algae Desmodesmus subspicatus. KCN, with a pKa of 9.11 at 30°C, is dissociated and protonated at the pH and temperature of the test conditions; thus, the active toxic principal is the cyanide ion.
Results Synopsis
Test Organism: Desmodesmus subspicatus (reported as Scenedesmus subspicatus)
Test Type: Static
96 hr LOEC: 0.16 mg active ingredient/L
Endpoint(s) Effected: Growth inhibition
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