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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in mammalian cells
Remarks:
Type of genotoxicity: gene mutation
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: valid scientific study

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1983
Reference Type:
publication
Title:
No information
Author:
O'Neill et al.
Year:
1977
Bibliographic source:
Mutation Research 45 91-101
Reference Type:
publication
Title:
No information
Author:
Snee and Irr.
Year:
1981
Bibliographic source:
Mutation Research 85 77-93

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
other: similar to OECD 476
Principles of method if other than guideline:
Method: other; similar to OECD 476
GLP compliance:
yes
Type of assay:
mammalian cell gene mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-hydroxy-2-methylpropionitrile
EC Number:
200-909-4
EC Name:
2-hydroxy-2-methylpropionitrile
Cas Number:
75-86-5
Molecular formula:
C4H7NO
IUPAC Name:
2-hydroxy-2-methylpropanenitrile
Details on test material:
IUCLID4 Test substance: as prescribed by 1.1 - 1.4

TS-Freetext:
98.5% Acetone cyanohydrin. Certificate of analysis.

Method

Target gene:
HPRT
Species / strain
Species / strain / cell type:
other: Chinese hamster cells CHO-K1-BH4
Metabolic activation:
with and without
Metabolic activation system:
Arocor-induced rat liver S9
Test concentrations with justification for top dose:
100 - 950 microgrammes/ml

Results and discussion

Test results
Key result
Species / strain:
Chinese hamster Ovary (CHO)
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
at 500 microgram/ml and higher
Vehicle controls validity:
valid
Untreated negative controls validity:
not examined
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'. Remarks: Chinese hamster cells CHO-K1-BH4

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative