2,2,2',2'-tetrakis(hydroxymethyl)-3,3'-oxydipropan-1-ol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

31 December 1991 and 2 January 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: control, 100 mg/L
- Sampling method: duplicate water samples were taken from the control and test replicates (R1 & R2 pooled and R3 & R4 pooled) at 0 and 48 hours.
- Sample storage conditions before analysis: The samples were stored frozen at -20°C until despatched to the sponsor for analysis.

Vehicle:

no

Details on test solutions:

Prepared by direct dispersion in water with the aid of ultrasonic disruption.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain: daphnia magna straus
- Source: Laboratory culture originating from a strain supplied by IRChA, France
- Age at study initiation (mean and range, SD): first instar

- Method of breeding: parthenogenesis; gravid adults were isolated 24 hours prior to initiation of the test. young daphnids produced overnight were used for testing.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

Not applicable

Hardness:

150 - 200 mg/L CaCO3.

Test temperature:

21°C

pH:

7.1

Dissolved oxygen:

8.6-8.9 mgO2/L

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal: 100 mg/L; Measured: 92mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: glass, 200 ml test solution
- Aeration: none
- No. of organisms per vessel:10
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):2
- Biomass loading rate: 20 mL test solution per organism


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: laboratory tap water, dechlorinated by passage through activated carbon, softened by reverse osmosis and "aged" by recirculation through a gravel filter bed.


OTHER TEST CONDITIONS
- Photoperiod: 16 h light: 8 hr dark


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilisation

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

Not applicable

Reported statistics and error estimates:

Not applicable

Validity criteria fulfilled:

yes

Conclusions:

Daphnia magna were exposed to a nominal exposure concentration of 0 and 100 mg/L dipentaerythritol for 48 hours under static conditions. The mean measured concentration of the 100 mg/L test treatment was 92 mg/L which indicated that the test medium had been adequately prepared, as the concentration was within +/- 20% the results of the study are based on the nominal exposure concentration. No immobility was observed during the test therefore, the 48 hr EC50 was >100 mg/L, the corresponding no-observed effect concentration (NOEC) was observed to be 100 mg/L.

Executive summary:

The 48-h acute toxicity of dipentaerythritol to Daphnia magna was studied under static conditions. 

Test organisms were exposed to dipentaerythtiol at nominal concentrations of 0 and 100 mg/L for 48 h. Solutions of dipentaerythritol were prepared by direct dispersion in water with the aid of ultrasonic disruption and were verified analytically at test initiation and test termination. The mean measured concentration of the 100 mg/L test treatment was 92 mg/L which indicated that the test medium had been adequately prepared. As the measured concentration was within +/- 20% of the nominal concentration the results of the study are based on the nominal exposure concentration. No immobility was observed during the test therefore, the 48 hr EC50 was >100 mg/L, the corresponding no-observed effect concentration (NOEC) was observed to be 100 mg/L.

This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates according to OECD guideline 202.

 

 

Results synopsis

Test organism age: < 24 h

Test type: Static

48-h NOEC: > 100 mg/L

48-h EC50: > 100 mg/L                 

Endpoint(s) effected: Immobilisation

 

Description of key information

Daphnia magna were exposed to a nominal exposure concentration of 0 and 100 mg/L dipentaerythritol for 48 hours under static conditions. The mean measured concentration of the 100 mg/L test treatment was 92 mg/L which indicated that the test medium had been adequately prepared, as the concentration was within +/- 20% the results of the study are based on the nominal exposure concentration. No immobility was observed during the test therefore, the 48 hr EC50 was >100 mg/L, the corresponding no-observed effect concentration (NOEC) was observed to be 100 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 100 mg/L