Adiponitrile

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

14 days

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Values were conducted using method of D.J Finney. Material was defined.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Materials and methods

Principles of method if other than guideline:

Method: Groups of 6 adult male rabbits were clipped free of hair over
the back and trunk area and fitted with plastic collars. The test
material (1000, 1250, 1350, 1500, 2000, or 4000 mg/kg) was
applied to intact skin on each rabbit under one gauze pad. The
trunk of each rabbit was then wrapped with a layer of plastic
wrap, stretch gauze bandage, and elastic adhesive tape. After
a 24-hour exposure period, the wrappings were removed, the
rabbits were wiped with a dry towel, and returned to their
cages. The rabbits were observed and/or weighed daily
(except weekends) over a 14-day recovery period and then
sacrificed.
The LD50 value was calculated from mortality data, using the method of D.J. Finney (Finney, D.J., Probit Analysis, 2nd Ed., 1952 Cambridge University Press.

GLP compliance:

no

Remarks:

GLP not followed

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Adiponitrile

EC Number:

203-896-3

EC Name:

Adiponitrile

Cas Number:

111-69-3

Molecular formula:

C6H8N2

IUPAC Name:

hexanedinitrile

Details on test material:

- Name of test material (as cited in study report): Adiponitrile; Hexanedinitriles; Hexanedioic acid dinitrile
- Other: CAS: 111-69-3

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male

Administration / exposure

Type of coverage:

occlusive

Vehicle:

not specified

Details on dermal exposure:

TEST SITE
- Area of exposure: back and trunk area
- Type of wrap if used: plastic wrap, strech gauze bandages and elastic adhesive tape.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): wipres with a dry towel

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1000 mg/kg, 1250 mg/kg, 1350 mg/kg, 1500 mg/kg, 2000 mg/kg, 4000 mg/kg.
- For solids, paste formed: no

Duration of exposure:

24 hours

Doses:

1000, 1250, 1350, 1500, 2000, or 4000 mg/kg

No. of animals per sex per dose:

6

Control animals:

not required

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Daily
- Other examinations performed: clinical signs: Intermittent weight loss at all levels tested. Gasping, diarrhea and lethargy at 4000 mg/kg. All deaths occurred within 2 days after dosing.

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

2 134 mg/kg bw

Mortality:

Mortality of 0/6, 0/6, 3/6, 4/6, 3/6, 4/6 was observed in the
1000, 1250, 1350, 1500, 2000, and 4000 mg/kg dose groups,
respectively. Intermittent weight loss occurred at all levels

Clinical signs:

other: respectively. Intermittent weight loss occurred at all levels tested. Gasping, diarrhea, and lethargy occurred at 4000 mg/kg. All deaths occurred within 2 days after dosing.

Applicant's summary and conclusion

Interpretation of results:

practically nontoxic

Remarks:

Migrated information >2000 mg/kg Criteria used for interpretation of results: OECD GHS

Conclusions:

2134 mg/kg dermal LD50 is above classification levels for toxic substances/preparations

Executive summary:

LD50 - ROUTE: Skin; DOSE: 2134 mg/kg/24H rabbit

Mortality of 0/6, 0/6, 3/6, 4/6, 3/6, 4/6 was observed in the

1000, 1250, 1350, 1500, 2000, and 4000 mg/kg dose groups,

respectively. Intermittent weight loss occurred at all levels

tested. Gasping, diarrhea, and lethargy occurred at

4000 mg/kg. All deaths occurred within 2 days after dosing.