Adiponitrile

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics

Type of information:

experimental study

Adequacy of study:

key study

Study period:

June 27, 1980 through June 30, 1980

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: This study was not conducted according to GLP and guidelines, but sufficient data are available for interpretation of results.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Objective of study:

absorption

distribution

excretion

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 417 (Toxicokinetics)

Deviations:

yes

Remarks:

Different in number of animals per group and in number of dose levels

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Adiponitrile

EC Number:

203-896-3

EC Name:

Adiponitrile

Cas Number:

111-69-3

Molecular formula:

C6H8N2

IUPAC Name:

hexanedinitrile

Details on test material:

As cited in study report, unlabeled adiponitrile was supplied by sponsor (Lot Fit 7865 BD-79-352). Adiponitrile (2,5 – 14C) lot #800105 was supplied by Pathfinder Laboratories Inc. The specific activity of 14C-adiponitrile was 1.5 m Ci/mM. The radiochemical purity of 14C-adiponitrile was determined on 2/27/81 by liquid chromatography using an Amino Spherisorb column (#20005). The mobile phase was hexane: dichloro-methane (3:1). A 50 ul aliquot of 14C-adiponitrile containing 17932 cpm was applied to the column. The total recovery of 14c-activity was 92%. The major peak of 14C-activity eluted in the 8-10 minute fraction. A minor peak of 14C-activity was also found eluting from 22-28 minutes. The major peak of 140-activity contained 76.3% of the 14C-activity recovered from the column. This analysis was performed eight months after receipt of the compound from Pathfinder Laboratories. It appears that the compound had decomposed after 8 months of storage at 0°C. Purity analysis of 14C-adiponitrile was also performed by Pathfinder Laboratories just prior to the study on May 20, 1980. The animals were dosed on June 24, 1980. A radiochromatogram scanner was used to detect 14C-labeled adiponitrile. The purity of 14C-adiponitrile was estimated to be greater than 92% just prior to dosing.

Radiolabelling:

yes

Remarks:

14C-adiponitrile

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

As cited in study report: Sprague-Dawley rats were received from Charles RiverBreeding Laboratories, Wilmington, Massachusetts 01877. The rats at dosing were 9 weeks (males) to 12 weeks (females) of age and ranged in weight from 200-300 g. Older rats were used because the rats were found to be below 200 g when they were 7-8 weeks old. The rats were ear-tagged for identification purposes. Rats were equilibrated a minimum of 12 days prior to dosing. The animals were fasted 15 hours prior to dosing to 4 hours post-dosing. After dosing the animals were housed individually in all glass metabolism cages for collection of urine, feces and expired air. Water (Elizabethtown Water Co.) was provided ad libitum and Purina Rodent Chow, No. 5001 milled, was supplied ad libitum before and after the fasting period. Expired air was trapped in Aquasol-2 to collect organic volatiles and in 1N sodium hydroxide bubblers to collect CO2.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Duration and frequency of treatment / exposure:

One single dose administration

Doses / concentrations

Remarks:

Doses / Concentrations:
One single dose, 5.75 mg/kg

No. of animals per sex per dose / concentration:

3 males and 3 females per dose

Control animals:

no

Positive control reference chemical:

no

Details on dosing and sampling:

One single dose was administered. Urine, feces, and expired air were collected at 0-8, 8-16, 16-24, 24-48, and 48-72 hours post-treatment.

Results and discussion

Toxicokinetic / pharmacokinetic studies

Details on absorption:

As cited in the study report: The cumulative 14C-activity found in the urine after 72 hours averaged 82.86% and 81.64% of the administered dose for males and females respectively. The cumulative 14C-activity found in feces averaged 5.83% and 6.66% of the administered dose for males and females, respectively. The total normalized 14C-activity found in the sodium hydroxide traps averaged 9.05% and 8.55% of the administered dose for males and females, respectively. The cumulative amount of 14C-activity found in the Aquasol-2 bubblers averaged 0.69% for the males and 0.21% for females of the administered dose.

Details on distribution in tissues:

Distribution in tissues was not determined because there was an average recovery of 97% from urine, feces, and expired air.

Transfer into organs

Observation:

not determined

Details on excretion:

As cited in the study report: The cumulative 14C-activity found in the urine after 72 hours averaged 82.86% and 81.64% of the administered dose for males and females respectively. The majority of the 14C-activity eliminated in the urine was found during the 0-8 and 8-16 hour intervals post-dosing. The cumulative 14C-activity found in feces averaged 5.83% and 6.66% of the administered dose for males and females, respectively. The majority of the 14C-activity was excreted into the feces during the 24-48 hour interval post-dosing. The total normalized 14C-activity found in the sodium hydroxide traps averaged 9.05% and 8.55% of the administered dose for males and females, respectively. The cumulative amount of 14C-activity found in the Aquasol-2 bubblers averaged 0.69% for the males and 0.21% for females of the administered dose.

Metabolite characterisation studies

Metabolites identified:

not measured

Details on metabolites:

not measured

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): no bioaccumulation potential based on study results
The data indicates that 14C-adiponitrile and its metabolites were quantitatively excreted 72 hours after oral administration. The major route of elimination appears to be via the kidney.

Executive summary:

Six Sprague-Dawley rats (3 male and 3 female) were administered a single dose of¹⁴C-adiponitrile by oral intubation. The dose contained approximately 1.38 mg of adiponitrile and 3.9 uCi of¹⁴C-adiponitrile. The dose averaged 5.75 mg/kg. Urine, feces, and expired air (1N NaOH bubblers for collecting¹⁴CO₂and Aquasol-2 for collecting volatile organics) were collected at 0-8, 8-16, 16-24, 24-48 and 48-72 hours post-treatment. The¹⁴C-activity found in the urine of 3 males and 3 females averaged 82.86% and 81.64%, respectively, of the administered dose. The¹⁴C-activity found in the feces averaged 5.83% and 6.66% of the administered dose it males and females, respectively. The¹⁴C- activity found in the normalized 1N NaOH bubblers averaged 9.05% in males and 8.55% in females of the administered dose. The¹⁴C-activity trapped by the Aquasol-2 bubblers in males and females averaged 0.69% and 0.21%, respectively, of the administered dose. The total¹⁴C-activity eliminated for male rats ranged from 80.96% to 117.27% with an average of 99.23%. The¹⁴C-activity eliminated from female rats ranged from 85.25% to 109.60% with an average recovery of 97.87%. The data indicates that¹⁴C-adiponitrile and its metabolites were quantitatively excreted 72 hours after oral administration. The major route of elimination appears to be via the kidney.