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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1987
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study but without GLP and deficiencies in documentation.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
not specified
Principles of method if other than guideline:
OECD 202 adopted 1984-04-04, part I
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
o-phenylenediamine
EC Number:
202-430-6
EC Name:
o-phenylenediamine
Cas Number:
95-54-5
Molecular formula:
C6H8N2
IUPAC Name:
benzene-1,2-diamine

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
HPLC was used
PLC (Dr. REusche, ZF-DZA-LEV/OAL); the test concentration 1,0 mg/l and 0,1 mg/l as well as the controlle were evaluated at the 03., 08., 18. February 1988 (directly after starting) and at the 5th, 10th, 12th of February 1988 (after 48 h testing) and as well at the 19.th of February (24 h after testing).

Test solutions

Vehicle:
no
Details on test solutions:
test concentration:
control
1.1 mg/l
4.4 mg/l
12.5 mg/l
50.0mg/l
70.7 mg/l
141.0 mg/l
200.0 mg/l

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
The Daphnia was sourced from Bundesgesundheitsamt Berlin, Klonzucht AWALU/Labor für Wassserbiologie
Breeding conditions: breeding was done in water bath under constant temperature (20 °C).
breeding water was conditioned tap water of Leverkusen
Feeding: with singular cell Scenedesmus subspicatus (out of fermenter breeding) ad libitum
before feeding ultrasound of 5 minutes, adapting of the algae suspension to extiguishing value of 0.54 at 578 nm in 1:10 solution
feeding of one adult per day: 0-1 day 0,10 ml feeding algae
2-3 day 0.12 ml feeding algae
4-5 day 0.16 ml feeding algae
6-7 day 0.20 ml feeding algae
8-20 day 0.40 ml feeding algae

measured and calcualted numbers during the test: Immobilisation and reproduction rates of the Daphnia

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h

Test conditions

Test temperature:
20 °C
pH:
ph was meassured at test start and after each new set-up of the test medium
Dissolved oxygen:
O2 was meassured at test start and after each new set-up of the test medium
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
0.8 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: (7.093 - 18.865)
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
11.9 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
200 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
Daphnia short term test
concentration number animals alive total number animals result (%)
0.8 mg/l 20 20 0
1.1 mg/l 18 20 10.00
4.4 mg/l 12 20 40.00
12.5 mg/l 10 20 50.00
50.0 mg/l 6 20 70.00
70.7 mg/l 4 20 80.00
141 mg/l 1 20 95.00
200 mg/l 0 20 100.00

EC05 = 0.642 (0.166 - 1.446) confidence level
EC10 = 1.223 (0.396 - 2.452)
EC50 = 11.86 (7.093 - 18.865)
EC90 = 115 (63.27 - 291.13)
EC95 = 219.29 (108.52 - 685.8839
Reported statistics and error estimates:
Test period 03. to 24 February 1988
HPLC (Dr. REusche, ZF-DZA-LEV/OAL); the test concentration 1,0 mg/l and 0,1 mg/l as well as the controlle were evaluated at the 03., 08., 18. February 1988 (directly after starting) and at the 5th, 10th, 12th of February 1988 (after 48 h testing) and as well at the 19.th of February (24 h after testing).

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
EC50 of the test item is 11.9 mg/l.
Executive summary:

EC50 of the test item is 11.9 mg/l.

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