o-phenylenediamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study but without GLP and deficiencies in documentation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

not specified

Principles of method if other than guideline:

OECD 202 adopted 1984-04-04, part I

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

o-phenylenediamine

EC Number:

202-430-6

EC Name:

o-phenylenediamine

Cas Number:

95-54-5

Molecular formula:

C6H8N2

IUPAC Name:

benzene-1,2-diamine

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

HPLC was used
PLC (Dr. REusche, ZF-DZA-LEV/OAL); the test concentration 1,0 mg/l and 0,1 mg/l as well as the controlle were evaluated at the 03., 08., 18. February 1988 (directly after starting) and at the 5th, 10th, 12th of February 1988 (after 48 h testing) and as well at the 19.th of February (24 h after testing).

Test solutions

Vehicle:

no

Details on test solutions:

test concentration:
control
1.1 mg/l
4.4 mg/l
12.5 mg/l
50.0mg/l
70.7 mg/l
141.0 mg/l
200.0 mg/l

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The Daphnia was sourced from Bundesgesundheitsamt Berlin, Klonzucht AWALU/Labor für Wassserbiologie
Breeding conditions: breeding was done in water bath under constant temperature (20 °C).
breeding water was conditioned tap water of Leverkusen
Feeding: with singular cell Scenedesmus subspicatus (out of fermenter breeding) ad libitum
before feeding ultrasound of 5 minutes, adapting of the algae suspension to extiguishing value of 0.54 at 578 nm in 1:10 solution
feeding of one adult per day: 0-1 day 0,10 ml feeding algae
2-3 day 0.12 ml feeding algae
4-5 day 0.16 ml feeding algae
6-7 day 0.20 ml feeding algae
8-20 day 0.40 ml feeding algae

measured and calcualted numbers during the test: Immobilisation and reproduction rates of the Daphnia

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test conditions

Test temperature:

20 °C

pH:

ph was meassured at test start and after each new set-up of the test medium

Dissolved oxygen:

O2 was meassured at test start and after each new set-up of the test medium

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

0.8 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: (7.093 - 18.865)

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

11.9 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

200 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Daphnia short term test
concentration number animals alive total number animals result (%)
0.8 mg/l 20 20 0
1.1 mg/l 18 20 10.00
4.4 mg/l 12 20 40.00
12.5 mg/l 10 20 50.00
50.0 mg/l 6 20 70.00
70.7 mg/l 4 20 80.00
141 mg/l 1 20 95.00
200 mg/l 0 20 100.00

EC05 = 0.642 (0.166 - 1.446) confidence level
EC10 = 1.223 (0.396 - 2.452)
EC50 = 11.86 (7.093 - 18.865)
EC90 = 115 (63.27 - 291.13)
EC95 = 219.29 (108.52 - 685.8839

Reported statistics and error estimates:

Test period 03. to 24 February 1988
HPLC (Dr. REusche, ZF-DZA-LEV/OAL); the test concentration 1,0 mg/l and 0,1 mg/l as well as the controlle were evaluated at the 03., 08., 18. February 1988 (directly after starting) and at the 5th, 10th, 12th of February 1988 (after 48 h testing) and as well at the 19.th of February (24 h after testing).

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

EC50 of the test item is 11.9 mg/l.

Executive summary:

EC50 of the test item is 11.9 mg/l.