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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1983
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline but without GLP and deficiencies in documentation.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1983
Report date:
1983

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
Principles of method if other than guideline:
The test was done due to the text of the method proposal (F.1.1.) "Letale Wirkung beim Zebrabärbling Brachydanio rerio" (LC0, LC50, LC100; 48-96 h) of the Umweltbundesamt, dated 1982-06-01
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
o-phenylenediamine
EC Number:
202-430-6
EC Name:
o-phenylenediamine
Cas Number:
95-54-5
Molecular formula:
C6H8N2
IUPAC Name:
benzene-1,2-diamine

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
body size 30 +/- 5 mm
10 animals per 3,5 l water

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Remarks on exposure duration:
without feeding
Post exposure observation period:
no data

Test conditions

Hardness:
3,5 l conditioned, via activated charcoal and a ion exchanger for Cu-removal sofetened drinking water with a Ca/Mg-range of 4:1 and a hardness of approx. 15 ° dH, at pH 7.0 +/- 0.2
Test temperature:
23 +/- 2 °C
pH:
7.0 +/- 0.2, after adding test material pH was 7.7
Dissolved oxygen:
air ventilation was done with 3 bubbles per second

Salinity:
Ca/Mg-range of 4:1 and a hardness of approx. 15 ° dH, at pH 7.0 +/- 0.2
Nominal and measured concentrations:
test substance added was 1000 mg/l
Details on test conditions:
size of test vessels: 8.4 l.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
18 mg/L
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
24 mg/L
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
32 mg/L
Basis for effect:
mortality (fish)
Details on results:
LC0, LC50 and LC100 are statistically calculated.



Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 of the test item determined in Brachydanio rerio was statistically calculated with 24 mg/l after 96 h.
Executive summary:

The LC50 of the test item determined in Brachydanio rerio was statistically calculated with 24 mg/l after 96 h.

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