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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Read-across from supporting substance: - Substance: Methyl acetate - CAS no.: 79-20-9 - EC no.: 201-185-2 Read-across justification: Methyl acetoacetate is a well defined mono-constitutent substance of high purity. Based on the similar structure and properties, the substance Methyl acetate was selected as representative model structure for read-across. Compared to MAA, the selected structure show similar physical-chemical, toxicological and ecotoxicological properties. A data matrix covering all available endpoints was previously published in the EU-Risk assessment Report Vol. 34 of 2003. The available data on MAA and selected model constituent revealed similar properties. In general, the classification and labeling of the model constituent is comparable to that of Methyl acetoacetate. Neither the model constituents nor MAA are considered PBT or vPvB substances. In summary, the read-across between MAA and the model constituent is justified. Result: Smyth et al screened industrial chemicals for acute toxicity values. For methylacetate the following values were determined: - LC0 = 49.2 mg/l/4h - LC50 = > 49.2 mg/l/4h

Data source

Reference
Reference Type:
publication
Title:
European Union Risk Assessment Report: METHYL ACETATE
Author:
European Union
Year:
2003
Bibliographic source:
EUR 20783 EN, Luxembourg: Office for Official Publications of the European Communities 2003 –VIII pp., 158 pp.

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Concentrated vapor inhalation consists of exposing 6 male or female albino rats to a following stream of vapor-loaded air. The vapor-air mixture is generated by passing 2.5L /minute of dried air at room temperature through a fritted glass disc immersed to a depth of at least one inch in approximately 50 ml of the test chemical contained in a gas-washing bottle. Inhalations are continued for time periods in a logarithmic series with a ratio of two extending from one-fourth to eight hours, until the inhalation period killing about half the number of rats within 14 days is defined. Inhalation of metered vapor concentrations by rats is conducted with flowing streams of vapor prepared by various styles of proportioning pumps. Inhalation periods are usually four hours' duration unless slight toxicity enforces an eight hour period. Concentrations recorded are nominal and not analytically verified. They are in an essentially logarithmic series with a factor of two, and the Table records the concentration yielding fractional mortality among 6 rats within 14 days. Where no fractional mortality was observed, usually both the concentration yielding no mortality and that yielding complete mortality are indicated.
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl acetate
EC Number:
201-185-2
EC Name:
Methyl acetate
Cas Number:
79-20-9
Molecular formula:
C3H6O2
IUPAC Name:
methyl acetate
Test material form:
not specified
Details on test material:
- Name of the test substance: Methyl acetate
- CAS No: 79-20-9
- E No: 201-185-2

Test animals

Species:
rabbit
Strain:
other: Albino
Sex:
male/female
Details on test animals or test system and environmental conditions:
no data

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: unchanged (no vehicle)
Details on inhalation exposure:
see test method description
Analytical verification of test atmosphere concentrations:
no
Duration of exposure:
4 h
Concentrations:
16,000 ppm and 32,000 ppm
No. of animals per sex per dose:
6 animals
Control animals:
no
Details on study design:
no data
Statistics:
no data

Results and discussion

Effect levelsopen allclose all
Dose descriptor:
LC0
Effect level:
49.2 mg/L air
Exp. duration:
4 h
Dose descriptor:
LC100
Effect level:
98.4 mg/L air
Exp. duration:
4 h
Dose descriptor:
LC50
Effect level:
> 49 mg/L air
Exp. duration:
4 h
Mortality:
No mortality observed
Clinical signs:
other: After inhalation of substance vapours, animals showed narcotic symptoms, spasms, dyspnea and vomiting; inhalation of vapours in addition caused irritation of eyes and upper respiratory tract.
Body weight:
no data
Gross pathology:
no data
Other findings:
no data

Any other information on results incl. tables

49.2 mg/l < LC50< 98.4 mg/l

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Read-across to Methyl acetate:
The acute inhalative LC50 (4hr) of Methyl acetate on Albino rabbits was determined to be > 49.2 mg/L air.
Executive summary:

Read-across from supporting substance:

- Substance: Methyl acetate

- CAS no.: 79 -20 -9

- EC no.: 201 -185 -2

Smyth et al screened industrial chemicals for acute toxicity values. For methylacetate the following values were determined:

- LC0 = 49.2 mg/l/4h

- LC50 = > 49.2 mg/l/4h