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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

toxicity to other aquatic vertebrates
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: non-standard test. For justification of read-across see CSR chapter 1.3.
Reason / purpose for cross-reference:
reference to same study

Data source

Reference Type:

Materials and methods

Test guideline
according to guideline
other: American Public Health Association 1975
not specified
Principles of method if other than guideline:
American Public Health Association 1975 Bioassays were performed under two different temperatures (22.7oC and 30.8oC) for 72-h in 500 mL beakers for plankton and for 96-h for other animals. Each container held 20 animals and six replicates were used for each test.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
calcium phosphoric acid
Cas Number:
Molecular formula:
calcium phosphoric acid
Constituent 2
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
not applicable
Details on test material:
- Name of test material (as cited in study report): superphosphate
- Composition of test material, percentage of components: N-P2O5-K2O, ratio 0-16-0
- Other: Commercial grade of superphosphate (N-P2O5-K2O, ratio 0-16-0) was used. No other details on the test material available

Sampling and analysis

Analytical monitoring:
not specified

Test organisms

Test organisms (species):
other: Moina micrura (zooplankton), Cyclops viridis (zooplankton), Branchiura sowerbyi (worm), Chironomus (insect larvae), Dragonfly nymph (insect larvae), Planorbis exustus (mollusc), Lymnaea leuteola (mollusc), Viviparus bengalensis (mollusc)

Study design

Test type:
not specified
Water media type:
not specified
Total exposure duration:
72 h
Remarks on exposure duration:
or 96 hours

Results and discussion

Any other information on results incl. tables

Avg.LC50(Moina micrura; 72-h) = 1,625 ppm superphosphate

Avg. LC50(Cyclops viridis; 72-h) = 2,305 ppm superphosphate

Avg. LC50(Branchiura sowerbyi; 96-h) = 3,320 ppm superphosphate

Avg. LC50(Chironomus; 96-h) = 1,510 ppm superphosphate

Avg. LC50(Dragonfly nymph; 96-h) = 1,133 ppm superphosphate

Avg. LC50(Planorbis exustus; 96-h) = 5,005 ppm superphosphate

Avg. LC50(Lymnaea leuteola; 96-h) = 2,950 ppm superphosphate

Avg. LC50(Viviparus bengalensis; 96-h) = 2,350 ppm superphosphate

Applicant's summary and conclusion

Executive summary:

A study for acute aquatic toxicity was performed with various invertebrate species. This study indicates that superphosphate is practically nontoxic to aquatic invertebrates. No significant difference in toxicity was observed at the two temperatures tested. Results are reported as the average of the two values for each species. All LC50 (72 resp. 96h) values found were above 1000 ppm.