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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study used for notification under 67/548. Original study report not available.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(phenylmethoxy)naphthalene
EC Number:
405-490-3
EC Name:
2-(phenylmethoxy)naphthalene
Cas Number:
613-62-7
Molecular formula:
C17H14O
IUPAC Name:
2-(benzyloxy)naphthalene
Test material form:
not specified
Details on test material:
no data

Method

Target gene:
no data
Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
Metabolic activation:
with and without
Metabolic activation system:
S9-Mix
Test concentrations with justification for top dose:
Concentration range in the main test (with metabolic activation): 312.5 ... 5000 µg/plate
Concentration range in the main test (without metabolic activation): 312.5 ... 5000 µg/plate
Vehicle / solvent:
Solvent: Aceton
Details on test system and experimental conditions:
no data
Evaluation criteria:
no data
Statistics:
no data

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
Cytotoxicity pretest:
no cytotoxicity up to doses of 5000 µg/plate (with and without metabolic activation)

Precipitation was not observed for the tested doses.
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

The results are conclusive but not sufficient for classification.
Executive summary:

The test article 2-(phenylmethoxy)naphthalene was assessed for its potential to induce gene mutations at the his locus using Salmonella typhimurium and at the trp locus using Escherichia Coli strains..

The study was performed with and without liver microsomal activation.

The test article was tested with the following concentrations:

312.5 ... 5000 µg/plate

No precipitation was observed for all concentrations tested with and without metabolic activation.

No Cytotoxicity was noted for all concentrations tested with and without metabolic activation.

No Mutagenicity was noted for all concentrations tested with and without metabolic activation.

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