1H-1,2,4-triazole, sodium salt

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From Aug. 18, 1992 to Sep. 24, 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study (OECD 401/423). No GLP study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Version / remarks:

year of guideline not reported

Deviations:

no

Test guideline 2

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Version / remarks:

reliability scoring based on 2001

Deviations:

no

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1H-1,2,4-triazole, sodium salt

EC Number:

255-280-9

EC Name:

1H-1,2,4-triazole, sodium salt

Cas Number:

41253-21-8

Molecular formula:

C2H3N3.Na

IUPAC Name:

sodium 1,2,4-triazol-1-ide

Details on test material:

- Name of test material (as cited in study report): 1, 2 , 4 - TRIAZOLE SODIUM SALT
- Physical state: white - yellow powder
- Analytical purity: = 99%
- Lot/batch No.: 92/492
- Expiration date of the lot/batch: after 1995 (received by laboratory on June 25, 1992)
- Storage condition of test material: in dark and at room temperature

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

no

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

other: 0.5% (w/v) methylcellulose in water

Details on oral exposure:

no

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5/sex/dose

Control animals:

no

Details on study design:

no

Statistics:

no data

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

< 2 000 mg/kg bw

Mortality:

9 rats in 10 died in less than 24 hours. 3 animals (2 males, 1 female) died between 6 and 7 hours after the start of the test. The following day, 6 rats (3 males, 3 females) were found dead. The only one surviving animal (female) was in a coma. No amelioration of its state was observed during the following 48 hours. The last one, moribund was sacrificed 3 days after the treatment.

Clinical signs:

other: One hour after the treatment, the spontaneous motor activity of all animals decreased. Some showed symptoms of respiratory difficulties (gasping). A few rats showed symptoms like piloerection and hollow side. The first severe signs of toxicity were observ

Gross pathology:

The necropsy carried out on all animals revealed stomach (meteorism, glandular mucosa diffusely red) and intestinal (small intestine distended and inflamed) changes.

Other findings:

no

Applicant's summary and conclusion

Conclusions:

Under the experimental conditions adopted, the oral LD50 of the substance 1,2,4-TRIAZOLE SODIUM SALT in male and female rats is smaller than 2000 mg/kg.

Executive summary:

Groups of 5 rats/sexe were exposed to 1,2,4 -triazole sodium salt by gavage at 2000 mg/kg bw, according to the OECD guideline. 1,2,4 -triazole sodium salt was dilued in 0.5%(w/v) methylcellulose in water. 9 rats in 10 died in less than 24 hours. Under the experimental conditions adopted, the oral LD50 of 1,2,4 -triazole sodium salt in male and female rats is smaller than 2000 mg/kg.