Tosyl chloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1978

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: A 2 page study study report. The study was performed pre-GLP. The study was performed according to a method similar to OECD401.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

Very high dose volume. Animals were only observed for signs of intoxication and necropsy was performed on surviving animals.

GLP compliance:

no

Remarks:

pre-GLP

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tosyl chloride

EC Number:

202-684-8

EC Name:

Tosyl chloride

Cas Number:

98-59-9

Molecular formula:

C7H7ClO2S

IUPAC Name:

4-methylbenzenesulfonyl chloride

Details on test material:

Name: para-toluene sulphochloride
Appearance: white-coloured crystalline powder

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

propylene glycol

Doses:

3.00, 3.60, 4.33, 5.18, 6.20 g/kg

No. of animals per sex per dose:

5

Control animals:

no

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

4 680 mg/kg bw

95% CL:

4 280 - 5 130

Any other information on results incl. tables

Results:

Within a few hours after dosing the rats showed sluggishness. Later on unconsciousness was frequently observed, especially in the two highest dose groups. Death occurred between 3 hours and 3 days after dosing. Thereafter the survivors recovered gradually and looked quite healthy again at the end of the observation period. Macroscopic examination of the survivors revealed no treatment-related gross alterations.

Dose		Mortality
Suspension ml/kg	Test substance g/kg	males	females	%
12.0	3.00	0/5	0/5	0
14.4	3.60	1/5	0/5	10
17.3	4.33	2/5	1/5	30
20.7	5.18	3/5	4/5	70
24.8	6.20	5/5	5/5	100

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Remarks:

Migrated information

Conclusions:

The LD50 of para-toluene sulphochloride was calculated to be 4.68 g per kg body weight with 4.28 and 5.13 as the 95 % confidence limits.
Therefore, the test substance can be classified in Category V according to GHS.

Executive summary:

An acute oral toxicity study was performed according to a method similar to OECD401 and pre-GLP. 5 male and 5 female rats were dosed with 3.00, 3.60, 4.33, 5.18 or 6.20 g/kg bw. The animals were observed for signs of intoxication during a 14-day period, after which autopsies were carried out on the survivors. Within a few hours after dosing the rats showed sluggishness. Later on unconsciousness was frequently observed, especially in the two highest dose groups. Death occurred between 3 hours and 3 days after dosing. Thereafter the survivors recovered gradually and looked quite healthy again at the end of the observation period. Macroscopic examination of the survivors revealed no treatment-related gross alterations.The LD50 of para-toluene sulphochloride was calculated to be 4.68 g per kg body weight with 4.28 and 5.13 as the 95 % confidence limits. Therefore, the test substance can be classified in Category V according to GHS.