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EC number: 941-809-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Solubility in organic solvents / fat solubility
Administrative data
Link to relevant study record(s)
- Endpoint:
- solubility in organic solvents / fat solubility
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- in March 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline 105, Method A6 of Commission Regulation (EC) No. 440/2008
- Version / remarks:
- 2008
- Deviations:
- yes
- Remarks:
- Due to lack of an official guideline for determination of octanol solubility, the procedure for the test was performed according to the procedure described in the guideline OECD 105, which is applied for determination of water solubility.
- GLP compliance:
- no
- Key result
- Medium:
- n-octanol
- Solubility:
- ca. 4.8 g/L
- Remarks on result:
- other: determined at room temperature
- Test substance stable:
- not determined
- Details on results:
- - Temperature effects: not reported
- Examination of different polarities: not applicable
- Information on chemical stability: not described
- for raw data, please see section "any other information on results incl. tables". - Conclusions:
- The solubility of the test item in n-octanol was determined to be 4.8 g/L.
- Executive summary:
In this study, the solubility of Sophorolipids: fermentation products of glucose and fatty acids, C18 (unsaturated), glycerol esters with yeast Candida, partially hydrolysed, in n-octanol was determined. Due to lack of an official guideline for determination of octanol solubility, the procedure for the test was performed according to the procedure described in guideline OECD Guideline 105, Method A6 of Commission Regulation (EC) No. 440/2008, which is applied for determination of water solubility.
The test item was stirred in n-octanol for 72 hours and then let equilibrate for 24 hours at room temperature before HPLC analysis was performed. A mixed phase in which the lactonic SLs contribute to the solubilization of acidic SLs in octanol was observed. Therefore, the octanol solubility of Sophorolipids is influenced by the composition of the active content in the sample used for test procedure.
The solubility of the test item in n-octanol was determined to be 4.8 g/L.
Reference
Due to the lack of reference substances a direct quantitative evaluation of the components in the HPLC/CAD analysis was not possible.
In order to provide an estimation of the acidic and lactonic SL content, an alternative approach was chosen by “evaluated as” calculations where all components are quantified applying the calibration function of one available reference compound. Thus, all identified acidic Sophorolipids components were quantified against a calibration function established for an acidic SL-reference material specifically synthesized, purified and characterized by NMR and HPLC.
A similar reference substance was prepared for the lactonic form, which was characterized for its SL-Lactone content by NMR and HPLC and which was used as calibration standard for all lactonic components.
The approach of quantifying the component groups against one typical representative can provide a reasonable quantitative estimation as it is our experience that the response of surfactant products at the CAD detection is mainly determined by the molecular structure but not primarily by chain length of the fatty acid.
Analytical results
Results of solubility of the test item in 1-octanol:
SL-acid [g/L] | SL-Lactone [g/L] | Sum [g/L] |
2.3 | 2.9 | 5.2 |
1.7 | 2.4 | 4.1 |
2.2 | 2.9 | 5.1 |
Average: 2.1 | Average: 2.7 | Average: 4.8 |
The composition of the obtained results does not reflect the composition of the original sample because lactonic Sophorolipids are better soluble in octanol than acidic Sophorplipids. Besides, it was observed the formation of a mixed phase in which the lactonic SLs contribute to the solubilization of acidic SLs in octanol. Therefore, the octanol solubility of Sophorolipids is influenced by the composition of the active content in the sample used for test procedure.
Description of key information
The solubility of the test item in n-octanol was determined to be 4.8 g/L.
Key value for chemical safety assessment
- Solubility in organic solvents at 20°C:
- 4.8 g/L
Additional information
The solubility of Sophorolipids: fermentation products of glucose and fatty acids, C18 (unsaturated), glycerol esters with yeast Candida, partially hydrolysed, in n-octanol was determined. Due to lack of an official guideline for determination of octanol solubility, the procedure for the test was performed according to the procedure described in guideline OECD Guideline 105, Method A6 of Commission Regulation (EC) No. 440/2008, which is applied for determination of water solubility.
The test item was stirred in n-octanol for 72 hours and then let equilibrate for 24 hours at room temperature before HPLC analysis was performed. A mixed phase in which the lactonic SLs contribute to the solubilization of acidic SLs in octanol was observed. Therefore, the octanol solubility of Sophorolipids is influenced by the composition of the active content in the sample used for test procedure.
The solubility of the test item in n-octanol was determined to be 4.8 g/L.
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