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EC number: 941-809-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Water solubility
Administrative data
Link to relevant study record(s)
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- in March 2017
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- For the cmc measurement no guideline could be identified. Yet for the actual study, measurement is documented with raw data (including diagrams), identification of equipment, description of measurement process, summary, identification of test substance. As a consequence the study and its documentation is sufficiently trustworthy to be used for risk assessment.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: accepted scientific principle
- Principles of method if other than guideline:
- The critical micelle concentration (cmc) was examined using tensiometry which represents an accepted scientific method. The surface tension of a surfactant solution does no longer change at the onset of cmc formation.
- GLP compliance:
- no
- Type of method:
- other: The cmc was determined using a tensiometer by plotting surface tension (mN/m) against concentration of the test item in solution (%). The cmc is regarded the relevant parameter for water solubility of surfactants.
- Key result
- Water solubility:
- >= 0.2 - <= 0.4 g/L
- Conc. based on:
- act. ingr.
- Remarks on result:
- other: critical micelle concentration
- Remarks:
- Temperature and pH not reported
- Details on results:
- The cmc of Sophorlipids is 0.02 – 0.04 % (active matter). Based on the assumption the density of the solution is 1 kg/L, this leads to a maximum solubility of Sophorolipids in water of 0.2 – 0.4 g/L.
- Conclusions:
- The critical micelle concentration (CMC) of the test item was determined to be 0.2 to 0.4 g/L.
- Executive summary:
In this study, the critical micelle concentration (cmc) of Sophorolipids: fermentation products of glucose and fatty acids, C18 (unsaturated), glycerol esters with yeast Candida, partially hydrolysed, was determined with a tensiometer by plotting surface tension (mN/m) against concentration of the test item in solution (%).
As a surfactant, the maximum solubility of Sophorolipids in water is derived from the critical micellar concentration. The cmc of Sophorolipids is 0.02 – 0.04 % (active matter). Based on the assumption the density of the solution is 1 kg/L, this leads to a maximum solubility of Sophorolipids in water of 0.2 – 0.4 g/L.
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- unknown
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- Version / remarks:
- adopted on 27 July 1995
- Deviations:
- yes
- Remarks:
- Guideline study with modifications.
- Qualifier:
- according to guideline
- Guideline:
- EU Method A.6 (Water Solubility)
- Deviations:
- yes
- Remarks:
- Guideline study with modifications.
- GLP compliance:
- no
- Type of method:
- flask method
- Key result
- Water solubility:
- >= 147 g/L
- Temp.:
- 23 °C
- pH:
- 5.3
- Conclusions:
- The test item remained clear when diluted with water. Consequently a water solubility of the test item can be defined as ≥ 147 g/L.
- Executive summary:
The solubility of the filtrated substance in water was determined according to OECD guidline 105 (adopted on 27 July 1995) with the flask method (modified) at 23 °C. The water solubility was checked visually. A value of >= 147 g/L was determined. It could be concluded that under the conditions of the test the substance was completely miscible with water.
- Endpoint:
- water solubility
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 2015-03-09 to 2015-03-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 105 (Water Solubility)
- Version / remarks:
- adopted on 27 July 1995
- Deviations:
- yes
- Remarks:
- Guideline study with modifications.
- Qualifier:
- according to guideline
- Guideline:
- EU Method A.6 (Water Solubility)
- Version / remarks:
- 30 May 2008
- Deviations:
- yes
- Remarks:
- Guideline study with modifications.
- GLP compliance:
- yes (incl. QA statement)
- Type of method:
- flask method
- Remarks:
- modified
- Conc. based on:
- test mat.
- Temp.:
- 24.7 °C
- pH:
- ca. 5.2
- Remarks on result:
- not determinable
- Remarks:
- The unfiltrated test substance is not completely miscible with water under the given conditions.
- Details on results:
- At the two tested concentrations of 94.2 g/L and 188.4 g/L, opalescent floating particles could be observed. They could be separated by filtration (syringe filter, nylon (PA), 0.20 µm; light blue). The resulting solutions were completely clear. It could be assumed that the test item contains insoluble as well as good water-soluble components. The results showed that the test item was in the tested concentrations and under the conditions of the test not completely miscible with water. For details please see table 1 in section “any other details on results incl. tables”.
- Conclusions:
- Under the given conditions, the unfiltrated test substance was not completely miscible with water.
- Executive summary:
The water solubility of the test item was determined according to OECD Guideline 105 [adopted on 27 July 1995] and EU test method A.6 [Council Regulation (EC) No 440/2008] with the modified flask method. The test item was not filtrated before the assay.
The unfiltrated test item is under the conditions of the test and in the tested concentration not completely miscible with water. Opalescent floating particles could be observed. It was assumed that the test item contains insoluble as well as good water-soluble components.
Water solubility (at 24.7 °C): not determinable.
Referenceopen allclose all
Surface tension of test item solutions at different concentrations | |
Concentration (% active matter) | Surface tension (mN/m) |
0.002 | 48 |
0.004 | 45 |
0.01 | 40 |
0.02 | 39 |
0.04 | 38 |
0.2 | 38 |
0.4 | 38 |
0.5 | 37 |
Sample no. |
Amount of sample [g] |
Amount of test item [g] |
Filled up with water to [mL] |
Test concentration [g/L]
|
Visual observations |
pH value1 |
1 |
2.017 |
0.734 |
5 |
147 |
clear sample |
5.3 |
1: The original sample had a pH value of 5.4
Table 1: Results
Sample No. |
Amount of test item [mL] |
Filled up with water to [mL] |
Test concentration [g/L] |
Visual observations |
pH value (1) |
1 |
2 |
10 |
94,2 |
Sample not clear; opalescent floating particles |
5.23 |
2 |
4 |
10 |
188.4 |
Sample not clear; opalescent floating particles |
5.22 |
(1) The purified water used for the tests had a pH value of 7.27, the original test item pH 5.31. Calculated with the density of the test item (determined by weighing of defined volumes): 471 mg/mL (in relation to water-free substance)
In both samples opalescent floating particles could be observed. They could be separated by filtration (syringe filter, nylon (PA), 0.20 um; light blue). The resulting solutions were completely clear.
Description of key information
In a first study, the unfiltrated test substance was not completely miscible with water. Therefore, in a subsequent study, the filtrated test substance was used and a solubility of ≥ 147 g/L was determined. The filtrated test substance was reported to be completely miscible with water. No turbidity was reported, but that the solution had remained clear.
As the test substance is a surfactant (surface tension was tested to be 37.3 nM/m), the critical micelle concentration (cmc) was determined since this is regarded the most relevant parameter for water solubility of substances with surface activity. The critical micelle concentration (cmc) of Sophorolipids: fermentation products of glucose and fatty acids, C18 (unsaturated), glycerol esters with yeast Candida, partially hydrolysed, was determined with a tensiometer by plotting surface tension (mN/m) against concentration of the test item in solution (%).
The cmc of Sophorolipids is 0.02 – 0.04 % (active matter). Based on the assumption the density of the solution is 1 kg/L, this leads to a maximum solubility of Sophorolipids in water of 0.2 – 0.4 g/L.
Key value for chemical safety assessment
- Water solubility:
- 0.4 g/L
- at the temperature of:
- 20 °C
Additional information
As the test substance is a surfactant (surface tension was tested to be 37.3 nM/m), the critical micelle concentration (cmc) was determined since this is regarded the most relevant parameter for water solubility of substances with surface activity. The critical micelle concentration (cmc) of Sophorolipids: fermentation products of glucose and fatty acids, C18 (unsaturated), glycerol esters with yeast Candida, partially hydrolysed, was determined with a tensiometer by plotting surface tension (mN/m) against concentration of the test item in solution (%). The cmc of Sophorolipids is 0.02 – 0.04 % (active matter). Based on the assumption the density of the solution is 1 kg/L, this leads to a maximum solubility of Sophorolipids in water of 0.2 – 0.4 g/L.
The solubility of the filtrated substance in water was determined according to OECD guidline 105 (adopted on 27 July 1995) with the flask method (modified) at 23 °C. The water solubility was checked visually. A value of >= 147 g/L was determined. It could be concluded that under the conditions of the test the substance was completely miscible with water.
As the test substance is surface active (surface tension was tested to be 37.3 nM/m), the critical micelle concentration (cmc) was determined in a preliminary study since this is regarded to be the most relevant parameter for water solubility of substances with surface activity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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