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EC number: 941-809-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2016-03-16 to 2016-04-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- 440/2008/EEC
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Sewage treatment plant Ruhrverband Kläranlage, Schmallenberg, mainly fed with municipal wastewater
- Concentration: 29.5 mg dry mass/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 60 mg/L
- Based on:
- test mat.
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
(a) KH2PO4: 8.5 g/L
K2HPO4: 21.75 g/L
Na2HPO4: 24.93 g/L
NH4Cl: 0.50 g/L
pH 7.4 +/- 0.2
(b) CaCl2 x 2H2O: 36.40 g/L
(c) MgSO4 x 7 H2O: 22.50 g/L
(d) FeCl3 x 6 H2O: 0.25 g/L
The mineral medium applied in the test contained 10 mL/L of mineral stock solution (a) and 1 mL/L of the mineral stock solutions (b) - (d), respectively.
- Solubilising agent (type and concentration if used): none
- Test temperature: 22 °C (+/-) 1 °C
- pH: 7.5-7.9
- pH adjusted: no
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration: Two vessels/concentration
- Method used to create aerobic conditions: Aeration during the whole test
- Measuring equipment: SAPROMAT respirometer (VOITH Inc.)
- Test performed in closed vessels
SAMPLING
- Sampling frequency: continuous measurement of the oxygen demand using a SAPROMAT respirometer
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: 100 mg test item/L sterilised mineral test medium (25mg/250 mL)
- Toxicity control: 100 mg test item/L and reference item at 100 mg/L mineral test medium (25 mg test item/250 mL and 25 mg reference item/250 ml) - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- The test item in concentrations of about 100 and 60 mg per litre mineral medium, respectively, and sodium benzoate in concentrations of about 100 mg per litre mineral medium, were incubated with 29.5 mg dry mass inoculum per litre mineral medium in 500 mL glass vessels at a medium volume of 250 mL. The test was run for 28 days, in darkness at 22 °C (+/-) 1 °C. The suspension was aerated during the whole test. Oxygen demand was carried out throughout the test course.
- Parameter:
- % degradation (O2 consumption)
- Value:
- 69
- St. dev.:
- 0.8
- Remarks on result:
- other: continous measurement
- Parameter:
- % degradation (O2 consumption)
- Value:
- 85
- St. dev.:
- 5.4
- Remarks on result:
- other: continous measurement
- Details on results:
- The biodegradation within the 10-day-window was 61 % and 75 % in the assays with 100 mg/L and 60 mg/L, respectively. The 10-day-window started at day 1 for a concentration of 60 mg/L and at day 2 for a concentration of 100 mg/L
- Results with reference substance:
- The degradation of the reference substance sodium benzoate had reached 83 % within the first 14 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The biodegradation of the test substance after 28 days incubation in the static test was found to be 69 % and 85 % in the assays with 100 mg/L and 60 mg/L, respectively.
- Executive summary:
The biodegradation of the test substance was investigated over a 28 day period in a manometric Respirometry Test according to OECD Guideline 301 F (1992). The test medium was inoculated with microorganisms from a digester of a sewage treatment plant mainly fed with municipal wastewater.
The rate of degradation was monitored by measuring the quantity of oxygen required to maintain a constant gas volume in the respirometer flasks over 28 days.
Sodium benzoate was used as a degradable reference substance at a concentration of 100 mg/L, along with a toxicity control at 100 mg/L test substance and 100 mg/L sodium benzoate.
The biodegradation of the test substance was found to be at mean 69 % with a standard deviation of 0.8 % for a concentration of 100 mg test substance/L and at mean 85 % with a standard deviation of 5.4 % for a concentration of 60 mg test item/L. For a concentration of 100 mg test substance/L biodegradation within the 10-day-window was found to be 61 %.
For a concentration of 60 mg test substance/L biodegradation within the 10-day-window was found to be 75 %.
The degradation of of the reference substance sodium benzoate had reached 83 % within the first 14 days.
According to the guideline, the test item is considered as being readily biodegradable under the test conditions.
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009 2009-02-19 to 2009-11-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 311 (Anaerobic Biodegradability of Organic Compounds in Digested Sludge: Measurement of Gas Production)
- Version / remarks:
- adopted 23 March 2006
- Deviations:
- yes
- Remarks:
- Several minor deviations which were considered irrelevant or uncritical for the result of the study
- Qualifier:
- according to guideline
- Guideline:
- other: EN ISO 11734
- Version / remarks:
- 1998
- Deviations:
- yes
- Remarks:
- Several minor deviations which were considered irrelevant or uncritical for the result of the study
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- anaerobic
- Inoculum or test system:
- anaerobic sludge
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Digester of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lache-Speyerdorf; batch number 25092009
- Pretreatment: The sludge was centrifuged and washed with anoxic test medium, then ventilated with oxygen-free Ar and re-suspended in anoxic test medium. A pre-fermentation was performed at 37 °C for five days. Sufficient washing was performed to reduce the content of inorganic carbon to < 12 mg/L. The sludge was diluted to give a concentration of solid in the tests of 3 g/L. Before tzhe start of the test the sludge was again ventilated with Ar. - Duration of test (contact time):
- 61 d
- Initial conc.:
- 397.7 mg/L
- Based on:
- test mat.
- Initial conc.:
- 172.4 mg/L
- Based on:
- act. ingr.
- Initial conc.:
- 407.7 mg/L
- Based on:
- test mat.
- Initial conc.:
- 176.8 mg/L
- Based on:
- act. ingr.
- Initial conc.:
- 396 mg/L
- Based on:
- test mat.
- Initial conc.:
- 171.7 mg/L
- Based on:
- act. ingr.
- Parameter followed for biodegradation estimation:
- other: Pressure of developed gas
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Potassium dihydrogene phosphate: 0.27 g
di-Sodium hydrogene phosphate-dihydrate: 0.555 g
Ammonium chloride: 0.53 g
Magnesium chloride hexahydrate: 0.1 g
Stock solution of trace elements: 10 mL
Iron-(II)-chloride tetrahydrate: 0.02 g
Resazurin (indicator for oxygen): 0.001 g
Sodiu sulfide: 0.1 g
deionised water: ad 1000 mL
- Test temperature: 35 (+/- 2) °C
- pH: 7.0 (+/- 0.2)
- pH adjusted: yes
TEST SYSTEM
-Inoculum concentration: 3 g suspended solids/L
- Volume of flasks: 500 mL
- Control flasks: 3 (medium and inoculum)
- Reference flasks: 3 (phenol, medium and inoculum)
- Test flasks: 3 (test substance, medium and inoculum)
- Toxicity control: 3 (test substance, phenol, medium and inoculum)
- Method used to create anaerobic conditions: The medium was prepared and purged with Ar. The gas was purged from oxygen using three washing bottles (first bottle with dithionite as oxygen acceptor, second bottle with pyrogallol as oxygen indicator, third bottle as liquid trap) The inoculum was taken from its source and prepared as described under "Details on inoculum".
On the day of the start of the test the stock solution for the positive control as well as fresh test medium were prepared and ventilated with Ar. The inoculum was diluted with medium to a concentration of solids of 3 g/L.
- Measuring equipment:
500 mL glass flakes with screw caps
OxyTop OC110 and measuring heads OxiTop-C
SAMPLING
No samples were taken; instead the pressure of the evolved gas in each flask was measured continously until the end of the test.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: yes - Reference substance:
- other: Phenol
- Key result
- Parameter:
- other: Pressure of developed gas
- Value:
- 110.7
- Remarks on result:
- other: Mean value from three vessels
- Details on results:
- The degree of biodegradation reached 100 %.
Lag-Phase: after 3 days, significant increase of pressure was detectable
Plateau-Phase: after 40 days, no significant pressure increase could be observed
Degradation at the end of the test: 100 % (calculated value 111 %)
The calculated degradation at the end of the test was more than 100 %. This might be due to the following: in the test flasks, bacterial growth was enhanced by the well biodegradable test item. After digestion of the test item, the biomass started self-digestion as no other organic nutrients were available. Self-digestion of the previously increased biomass in the test flasks might very well been higher than in the control flasks. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test substance was readily biodegradable under anaerobic conditions.
- Executive summary:
The anaerobic biodegradation of the test substance was determined in a test according to OECD guideline 311 (2006) and EN ISO 11734 (1998). Biodegradation was monitored by continuous measurement of the gas developed until the end of the test. Anaerobic sludge was used as inoculum.
After 61 days a degradation of 110.7 % was observed. The degradation exceeding 100 % might be explained by the following: In the test flasks bacterial growth was enhanced by the well biodegradable test substance. After digestion of the test substance the biomass started self-digestion. Self-digestion of the previously increased biomass in the test flasks might very well be higher than the control flasks.
Except for the degradation of the positive control, the validity criteria were met. As the test item was readily and completely biodegradable under test conditions, this deviation from the guideline can be stated as uncritical.
Therefore, the test item is stated to be biodegradable under anaerobic conditions.
Referenceopen allclose all
The biodegradation of the test item after 28 days of incubation in the static test was found to be 69 % (SD = 0.8 %) and 85 % (SD = 5.4 %) in the assays with 100 mg/L and 60 mg/L, respectively. The biodegradation within the 10-day-window was 61 % and 75 % in the assays with 100 mg/L and 60 mg/L, respectively. The 10-day-window started at day 1 for a concentration of 60 mg test item per litre and at day 2 for a concentration of 100 mg test item per litre.
With 4 %, there was no significant abiotic degradation of the test item noticeable within the 28 days of incubation.
The biodegradation of the item mixture in the toxicity control was found to be 65 % after 14 days of incubation. Thus, the demanded threshold value of 25 % is exceeded and the test item can be identified as non-toxic in a ready biodegradability test.
The reference item sodium benzoate was degraded to 83 % within the first 14 days.
Table 1: pH values at test start. Single values of the parallel test vessels.
vessel |
Inoculum blank |
Test suspension A |
Test suspension B |
Abiotic control |
Procedural control |
Toxicity control |
1 |
7.5 |
7.5 |
7.5 |
7.5 |
7.5 |
7.5 |
2 |
7.6 |
7.5 |
7.5 |
7.5 |
7.5 |
7.5 |
Table 2: pH values at test end. Single values of the parallel test vessels.
vessel |
Inoculum blank |
Test suspension A |
Test suspension B |
Abiotic control |
Procedural control |
Toxicity control |
1 |
7.7 |
7.9 |
7.9 |
7.7 |
8.5 |
8.8 |
2 |
7.8 |
7.9 |
7.9 |
7.7 |
8.6 |
8.8 |
Table 3: Oxygen consumption. Cumulated consumption (mg O2/L) after 14 days. Single and mean values of the parallel test vessels and standard deviation. Test suspension B: 60 mg/L; Test suspension A and abiotic control: 100 mg/L; Procedural control: 100 mg/L; Toxicity control: 200 mg/L.
vessel |
Inoculum blank |
Test suspension A |
Test suspension B |
Abiotic control |
Procedural control |
Toxicity control |
1 |
22 |
76 |
59 |
0 |
160 |
189 |
2 |
23 |
77 |
67 |
3 |
161 |
187 |
Mean |
23 |
77 |
63 |
2 |
161 |
188 |
SD |
1 |
1 |
6 |
2 |
1 |
1 |
Table 4: Percent degradation. Degradation (%) after 14 days. Single and mean values of the parallel test vessels and standard deviation.
vessel |
- |
Test suspension A |
Test suspension B |
Abiotic control |
Procedural control |
Toxicity control |
1 |
- |
61.8 |
70.3 |
0.0 |
82.6 |
65.8 |
2 |
- |
63.0 |
85.7 |
3.5 |
83.2 |
65.0 |
Mean |
- |
62.4 |
78.0 |
1.7 |
82.9 |
65.4 |
SD |
- |
0.8 |
10.9 |
2.5 |
0.4 |
0.6 |
Table 5: Per Oxygen consumption. Cumulated consumption (mg O2/L) after 28 days. Single and mean values of the parallel test vessels and standard deviation. Test suspension B: 60 mg/L; Test suspension A and abiotic control: 100 mg/L; Procedural control: 100 mg/L; Toxicity control: 200 mg/L. cent degradation. Degradation (%) after 14 days. Single and mean values of the parallel test vessels and standard deviation.
vessel |
Inoculum blank |
Test suspension A |
Test suspension B |
Abiotic control |
Procedural control |
Toxicity control |
1 |
28 |
88 |
70 |
0 |
172 |
204 |
2 |
28 |
87 |
74 |
7 |
174 |
205 |
Mean |
28 |
88 |
72 |
4 |
173 |
205 |
SD |
0 |
1 |
3 |
5 |
1 |
1 |
Table 6: Percent degradation. Degradation (%) after 28 days. Single and mean values of the parallel test vessels and standard deviation.
vessel |
- |
Test suspension A |
Test suspension B |
Abiotic control |
Procedural control |
Toxicity control |
1 |
- |
69.3 |
80.9 |
0.0 |
86.5 |
69.5 |
2 |
- |
68.2 |
88.6 |
8.1 |
87.7 |
69.9 |
Mean |
- |
68.7 |
84.7 |
4.0 |
87.1 |
69.7 |
SD |
- |
0.8 |
5.4 |
5.7 |
0.8 |
0.3 |
Carbon content in the gas phase at the end of the test
Emitted carbon in the gas phase in mg was calculated using the following equation:
Mh= 0.468 x Δpkum x Vh
with
mh= net weight of emitted carbon in the gas phase in mg
Δpkum= cumulated gas pressure test minus mean gas pressure control:
pT(1) – pBI(1) + pT(2) + …pT(n) – pBI(n)
Flask |
Pressure |
Net Pressure |
Gas phase |
Net carbon content Gas Phase |
Designation |
hPa |
hPa |
L |
mg |
Control 1 |
84 |
|
0.1310 |
-- |
Control 2 |
72 |
|
0.1192 |
-- |
Control 3 |
54 |
|
0.1191 |
-- |
Control mean |
70 |
|
|
-- |
Pos. Control 1 |
90 |
20 |
0.1295 |
1.2 |
Pos. Control 2 |
86 |
16 |
0.1023 |
0.8 |
Pos. Control 3 |
84 |
14 |
0.1101 |
0.7 |
Pos. Control Mean |
87 |
17 |
|
0.9 |
Test 1 |
850 |
780 |
0.1016 |
37.1 |
Test 2 |
846 |
776 |
0.1160 |
42.1 |
Test 3 |
878 |
808 |
0.1012 |
38.3 |
Test Mean |
858 |
788 |
|
39.2 |
Toxicity control 1 |
771 |
701 |
0.1225 |
40.2 |
Toxicity control 2 |
871 |
801 |
0.1251 |
46.9 |
Toxicity control 3 |
953 |
883 |
0.1271 |
52.5 |
Toxicity control Mean |
865 |
795 |
|
46.5 |
Inorganic carbon content in the liquid phase in mg
The carbon content in the liquid phase is is calculated using the following equation:
mi = IC(Test) x Vf(Test) – Σ3i-1[IC(Control)I x Vf(Control)i]/3
with
mi = inorganic carbon content in the liquid phase in mg
IC(Test) x Vf(Test) = inorganic carbon in the respective test flask in mg
Σ3i-1[IC(Control)I x Vf(Control)i]/3 = mean inorganic carbon in the control flasks in mg
Vf= volume of the liquid phase in the respective glass in litre
Flask |
Measured inorganic carbon content |
Volume liquid phase |
Inorganic carbon in flask (abs.) |
Net inorganic carbon content liquid phase |
Designation |
mg/L |
L |
mg |
mg |
Control 1 |
25.83 |
0.4912 |
12.69 |
|
Control 2 |
23.52 |
0.4879 |
11.48 |
|
Control 3 |
23.41 |
0.4967 |
11.63 |
|
Control mean |
24.68 |
|
11.93 |
|
Pos. Control 1 |
29.73 |
0.4926 |
14.64 |
2.71 |
Pos. Control 2 |
26.25 |
0.5026 |
13.19 |
1.26 |
Pos. Control 3 |
26.32 |
0.507 |
13.34 |
1.41 |
Pos. Control Mean |
27.43 |
|
|
1.80 |
Test 1 |
59.53 |
0.5167 |
30.76 |
18.83 |
Test 2 |
47.38 |
0.5087 |
24.10 |
12.17 |
Test 3 |
59.35 |
0.5134 |
30.47 |
18.54 |
Test Mean |
55.42 |
|
|
16.51 |
Toxicity control 1 |
41.62 |
0.4997 |
20.80 |
8.87 |
Toxicity control 2 |
53.95 |
0.4864 |
26.24 |
14.31 |
Toxicity control 3 |
63.57 |
0.5004 |
31.81 |
19.88 |
Toxicity control Mean |
53.05 |
|
|
14.35 |
Elimination carbon in mg
The total eliminated carbon is calculated by addition of the two values.
mt= mh+ mi
with mt= carbon which was transformed to CH4or CO2
Flask |
Carbon content gas phase |
Carbon content liquid phase |
Total eliminated carbon |
Designation |
mg |
mg |
mg |
Pos. Control 1 |
1.2 |
2.71 |
3.9 |
Pos. Control 2 |
0.8 |
1.26 |
2.0 |
Pos. Control 3 |
0.7 |
1.41 |
2.1 |
Pos. Control Mean |
0.9 |
1.80 |
2.71 |
Test 1 |
37.1 |
18.83 |
55.92 |
Test 2 |
42.1 |
12.17 |
54.31 |
Test 3 |
38.3 |
18.54 |
56.82 |
Test Mean |
39.2 |
16.51 |
55.68 |
Toxicity control 1 |
40.2 |
8.87 |
49.06 |
Toxicity control 2 |
46.9 |
14.31 |
61.22 |
Toxicity control 3 |
52.5 |
19.88 |
72.41 |
Toxicity control Mean |
46.5 |
14.35 |
60.90 |
Degree of elimination
The degradation is calculated using the following equation:
Di = mt x 100 / mz
with
Dt = grade of elimination in %
mt = carbon which was transformed to CH4 or CO2 in mg
mz = organic carbon which was originally added to the test flasks in mg
Flask |
Total eliminated carbon |
Added C in resp. flask |
Degradation |
Designation |
mg |
mg |
% |
Pos. Control 1 |
3.9 |
48.9 |
8.07 |
Pos. Control 2 |
2.0 |
48.9 |
4.18 |
Pos. Control 3 |
2.1 |
48.9 |
4.40 |
Pos. Control Mean |
|
|
5.55 |
Test 1 |
55.92 |
50.3 |
111.2 |
Test 2 |
54.31 |
50.8 |
106.9 |
Test 3 |
56.82 |
49.8 |
114.1 |
Test Mean |
|
|
110.7 |
Toxicity control 1 |
49.06 |
98.5 |
49.8 |
Toxicity control 2 |
61.22 |
98.8 |
62.0 |
Toxicity control 3 |
72.41 |
99.3 |
72.9 |
Toxicity control Mean |
|
|
61.6 |
Description of key information
The biodegradation of the substance was investigated in tests under aerobic and under anaerobic conditions. Two studies on the target substance proved that the substance is readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
The biodegradability of Sophorolipids was investigated in three guideline studies.
a. OECD guideline 301F
The biodegradation of the test substance was investigated over a 28 day period in a manometric Respirometry Test according to OECD Guideline 301 F (1992). The test medium was inoculated with microorganisms from a digester of a sewage treatment plant mainly fed with municipal wastewater.
The rate of degradation was monitored by measuring the quantity of oxygen required to maintain a constant gas volume in the respirometer flasks over 28 days.
Sodium benzoate was used as a degradable reference substance at a concentration of 100 mg/L, along with a toxicity control at 100 mg/L test substance and 100 mg/L sodium benzoate. The biodegradation of the test substance was found to be at mean 69 % with a standard deviation of 0.8 % for a concentration of 100 mg test substance/L and at mean 85 % with a standard deviation of 5.4 % for a concentration of 60 mg test item/L. For a concentration of 100 mg test substance/L biodegradation within the 10-day-window was found to be 61 %.
For a concentration of 60 mg test substance/L biodegradation within the 10-day-window was found to be 75 %.
The degradation of of the reference substance sodium benzoate had reached 83 % within the first 14 days.
According to the guideline, the test item is considered as being readily biodegradable under the test conditions.
b. OECD guideline 311
The anaerobic biodegradation of the test substance was determined in a test according to OECD guideline 311 (2006) and EN ISO 11734 (1998). Biodegradation was monitored by continuous measurement of the gas developed until the end of the test. Anaerobic sludge was used as inoculum.
After 61 days a degradation of 110.7 % was observed. The degradation exceeding 100 % might be explained by the following: In the test flasks bacterial growth was enhanced by the well biodegradable test substance. After digestion of the test substance the biomass started self-digestion. Self-digestion of the previously increased biomass in the test flasks might very well be higher than the control flasks.
Except for the degradation of the positive control, the validity criteria were met. As the test item was readily and completely biodegradable under test conditions, this deviation from the guideline can be stated as uncritical.
Therefore, the test item is stated to be biodegradable under anaerobic conditions.
c. Read-across study, OECD 301C test
The biodegradation of the test substance was determined in an OECD 301C test (modified MITI test). The degradation was measured by means of oxygen uptake. After 28 days more than 99 % of the test substance was degraded, meeting the 10 day window requirement. Under the conditions of the test the test substance was readily biodegradable.
Discussion
The test item Sophorolipids was readily biodegradable in both an OECD guideline 301F and an OECD guideline 311 test.
A supporting study based on a read-across approach is available which gave comparable results.Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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