2-methylbutyl acrylate

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vitro / ex vivo

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

25 Jan 2001 to 30 Jan 2001

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Read-across of data for an analogous chemical.The target chemical and the source chemical are very closely related alkyl acrylate compounds. They differ only by the addition of a methyl group in the butyl portion of the molecule. Both molecules are expected to be metabolized via the same hydrolysis and enzymatic pathways. The data from the source chemical are experimental results from well documented studies which meet basic scientific principles and are considered to be acceptable with restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Objective of study:

metabolism

Principles of method if other than guideline:

n-Butyl acrylate was tested for relative rates of hydrolysis by porcine hepatic esterase.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

n-Butyl acrylate

IUPAC Name:

n-Butyl acrylate

Constituent 2

Reference substance name:

Butyl acrylate

EC Number:

205-480-7

EC Name:

Butyl acrylate

Cas Number:

141-32-2

IUPAC Name:

butyl acrylate

Details on test material:

- Name of test material (as cited in study report): n-Butyl acrylate
- Substance type: liquid
- Analytical purity: 99.66%
- Lot/batch No.: F0311-01-GB

Radiolabelling:

no

Administration / exposure

Duration and frequency of treatment / exposure:

2 min, 5 min and 3 hours

Doses / concentrations

Remarks:

Doses / Concentrations:
0.2, 0.5 and 2 mM

Results and discussion

Toxicokinetic / pharmacokinetic studies

Toxicokinetic parameters

Toxicokinetic parameters:

other: n-Butyl acrylate was rapidly hydrolyzed at rates ranging between 54-69 µmoles/min/mg protein (4 - 33 %) after 2 minutes and 43-72 µmoles/min/mg protein (10 - 66 %) after 5 minutes of incubation at 37°C.

Metabolite characterisation studies

Metabolites identified:

no

Any other information on results incl. tables

The % conversion results of n-Butyl acrylate (an average of 3 determinations)

Concentration (mM)	% Conversion / Rate*
	At 2 min.	At 5 min.
2.00	4.18 / 69.67	10.09 / 67.27
0.50	18.16 / 75.67	43.72 / 72.87
0.20	32.80 / 54.67	65.97 / 43.98

* in μmoles / min / mg protein

n-Butyl acrylate was rapidly hydrolyzed at rates ranging between 54-69 µmoles/min/mg protein (4 - 33 %) after 2 minutes and 43-72 µmoles/min/mg protein (10 - 66 %) after 5 minutes of incubation at 37°C.

Applicant's summary and conclusion

Executive summary:

The results presented in this summary are experimental results for the source chemical n-butyl acrylate. The results are considered to be appropriate for read-across to the target chemical 2-methylbutyl acrylate.