Registration Dossier
Registration Dossier
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EC number: 700-420-0 | CAS number: 7605-52-9
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
Ames test: It is concluded that M3MC-Carboxylate showed no evidence of mutagenic activity in this bacterial system under the test conditions employed.
Chromosome Aberration test: It is concluded that the test substance M3MC-Carboxylate has shown no evidence of causing an increase in the frequency of structural chromosome aberrations in this in vitro cytogenetic test system, under the experimental conditions described.
Short description of key information:
Two tests for in vitro toxicity have been carried out, an Ames test and a chromosome aberration test.
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
Ames test: Concentrations of M3MC-Carboxylate up to 5000 µg/plate were tested. This is the standard limit concentration recommended in the regulatory guidelines that this assay follows. Other concentrations used were a series of ca half-log10dilutions of the highest concentration. No signs of toxicity were observed towards the tester strains in the first mutation test following exposure to M3MC-Carboxylate, but toxicity, observed as a slight thinning of the background lawn of non-revertant colonies, was obtained in the second mutation test following exposure to M3MC-Carboxylate at 5000 µg/plate in the absence of S9 mix.
No evidence of mutagenic activity was seen at any concentration of M3MC-Carboxylate in either mutation test.
Chromosome aberration test: in both the absence and presence of S9 mix, M3MC-Carboxylate caused no statistically significant increases in the proportion of metaphase figures containing chromosomal aberrations, at any dose level, when compared with the solvent control, in either test.
No statistically significant increases in the proportion of polyploid cells were seen, in either test.
All positive control compounds caused statistically significant increases in the proportion of aberrant cells, demonstrating the sensitivity of the test system and the efficacy of the S9 mix.
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