Registration Dossier
Registration Dossier
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EC number: 700-420-0 | CAS number: 7605-52-9
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: EC Directive 88/302, Method C.11, ‘Biodegradation - Activated Sludge Respiration Inhibition Test’ and OECD Test Guideline 209, ‘Activated Sludge, Respiration Inhibition Test’.
- GLP compliance:
- yes
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
Study design
- Test type:
- static
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 210 mg/L
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 105 mg/L
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- 418 mg/L
Applicant's summary and conclusion
- Conclusions:
- Concentration-related inhibition of respiration rates were observed at concentrations between 100 and 1000 mg/L, with the highest concentration (triplicate,1000 mg/L) showing 94 to 96% inhibition.
The three-hour EC50 for M3MC-Carboxylate was calculated to be 210 mg/L (95% confidence limits, 176 – 248 mg/L). The EC20 and EC80 were calclated to be 105 and 418 mg/L (95% confidence limits, 78 – 141 mg/L and 339 – 520 mg/L), respectively.
The three-hour EC50 for 3,5-DCP (6.6 mg/L) fulfilled the validity criterion relating to sensitivity to inhibition (acceptable EC50 range 5 to 30 mg/L), and that relating to the respiration rates in the control (variation not greater than 15%) was also satisfied. - Executive summary:
Concentration-related inhibition of respiration rates were observed at concentrations between 100 and 1000 mg/L, with the highest concentration (triplicate,1000 mg/L) showing 94 to 96% inhibition. The three-hour EC50 for M3MC-Carboxylate was calculated to be 210 mg/L (95% confidence limits, 176 – 248 mg/L). The EC20 and EC80 were calculated to be 105 and 418 mg/L (95% confidence limits, 78 – 141 mg/L and 339 – 520 mg/L), respectively. The three-hour EC50 for 3,5-DCP (6.6 mg/L) fulfilled the validity criterion relating to sensitivity to inhibition (acceptable EC50 range 5 to 30 mg/L), and that relating to the respiration rates in the control (variation not greater than 15%) was also satisfied.
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