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Short-term toxicity to fish

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Description of key information

The acute toxicity to fish (danio rerio) of the test substance was tested in a static test. After 96 hours of exposure no effect against fish were observed at a limit test concentration of 100 mg/L (Neuhahn, 2012).

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
100 mg/L

Additional information

A study was performed to assess the acute toxicity of the test substance to Danio rerio under static conditions. The study was conducted in accordance with Council Regulation (EC) No 440/2008, Method C.1 'Acute toxicity for Fish' (2008) which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 203 'Fish, Acute Toxicity Test' (1992).

An acute daphnia range finding toxicity test preceded the fish test and provided information about the range of concentrations which were used in the fish test. The following nominal concentration of the test item was tested in the daphnia range finding test: 100 mg/L.

Because of the anticipated low toxicity of the test item, fish were exposed in the fish test to a limit test concentration of nominally 100 mg/L for a period of 96 hours. As no toxic effects against fish were observed, no statistical analysis was required to determine the LC 50. Additionally any abnormal behavior or appearance of the fish was reported every 24 hours.

After 96 hours of exposure a LC50 of >100 mg/L (nominal) was determined.

This toxicity study is classified acceptable and satisfies the guideline requirements for the acute fish.

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