2,3-dichloroaniline

Biodegradation in water and sediment: simulation tests

Administrative data

Endpoint:

biodegradation in water: sediment simulation testing

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

Reductive Dehalogenation of Chloroanilines in Anaerobic Estuarine Sediment

Author:

Susarla S, Yonezawa Y, Masunaga S

Year:

1997

Bibliographic source:

Environmental Technology 18: 75 - 83

Materials and methods

Principles of method if other than guideline:

other: no guideline followed; see Test Conditions

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2,3-dichloroaniline

EC Number:

210-157-9

EC Name:

2,3-dichloroaniline

Cas Number:

608-27-5

Molecular formula:

C6H5Cl2N

IUPAC Name:

2,3-dichloroaniline

Details on test material:

Name of test material (as cited in publication): 2,3-DCA.
No purity stated.
The test material was purchased from Tokyo Kasei Kogyo Co. Ltd., (TCI), Tokyo, Japan and were used without further purification.
Stock solution: 2x10^-3 moles/L in methanol.

Radiolabelling:

no

Study design

Oxygen conditions:

anaerobic

Inoculum or test system:

natural sediment

Details on source and properties of sediment:

The sediment used in the experiment was collected at the mouth of Tsurumi River Japan. The surface sediment sample was collected by an Ekman-Barge sediment sampler from the Tsurumi-Ohashi bridge in September 1992.
The sediment was black in color indicating sulfate reducing activity.
Macro benthic organisms, such as crabs, small fish and shellfish were present in the collected sediment.
The sediment was sieved through a 2 mm screen to remove debris.
The sieved sediment sample was mixed with river water to make sediment slurry with a solids concentration of 272 +/- 2.8 g/Kg.
This was an average of four replicate measurements.
The pH of the sediment slurry was 5.6.

Duration of test (contact time):

1 yr

Initial test substance concentration

Initial conc.:

4 µmol/L

Based on:

test mat.

Details on study design:

- for each replicate a 5mL of sediment slurry was placed in a screw top test tube and sealed under N2 atmosphere.
- the tubes were sealt and kept at room temperature for one week to ensure that the system was anaerobic.
- all test tubes were placed in an anaerobic chamber (10% H2, 10% CO2, 80% N2)
- 10 µL of stock solution was added to each test tube to give an initial concentration of 4 µmoles/L
- immediately after spiking, time zero (Co) samples were frozen at -20 °C, while other test tubes were kept in a constant temperature room at 25 °C
- the slurries were hand mixed three times a week by turning upside down for few minutes
- the test tubes were sampled once a day at the beginning of the incubation then at longer time intervals as the experiment progressed
- the incubation lasted for a year

Results and discussion

% Degradation

% Degr.:

ca. 98

Parameter:

test mat. analysis

Sampling time:

1 yr

Half-life of parent compound / 50% disappearance time (DT50)

Compartment:

sediment

DT50:

54 d

Type:

not specified

Other kinetic parameters:

first order rate constant

Transformation products:

yes

Identity of transformation products

Identity of transformation products 1

No.:

#1

Reference

Reference substance name:

Unnamed

IUPAC name:

3-chloroaniline

Inventory number:

InventoryMultipleMappingImpl [inventoryEntryValue=EC 203-581-0]

CAS number:

108-42-9

Identity:

Benzenamine, 3-chloro-

Molecular formula:

C6H6ClN

Molecular weight:

127.572

SMILES notation:

Nc1cccc(Cl)c1

InChl:

InChI=1/C6H6ClN/c7-5-2-1-3-6(8)4-5/h1-4H,8H2

Identity of transformation products 2

No.:

#2

Reference

Reference substance name:

Unnamed

IUPAC name:

aniline

Inventory number:

InventoryMultipleMappingImpl [inventoryEntryValue=EC 200-539-3]

CAS number:

62-53-3

Molecular formula:

C6H7N

Molecular weight:

93.126

SMILES notation:

Nc1ccccc1

InChl:

InChI=1/C6H7N/c7-6-4-2-1-3-5-6/h1-5H,7H2