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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented report which meets basic scientific principles

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report date:
1976

Materials and methods

Principles of method if other than guideline:
15 rats/sex received a singel oral application per gavage of 250, 500, 1000, 1500, 2000, 2500, 2500 3000, 3500 or 5000 mg/kg bw - male rates resp. 500, 1000, 1500, 2000, 2500, 3000, 3250 or 3500 mg/kg bw - female rats, of the test substance. Post-exposure period was 14 days.
GLP compliance:
no
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
2,3-dichloroaniline
EC Number:
210-157-9
EC Name:
2,3-dichloroaniline
Cas Number:
608-27-5
Molecular formula:
C6H5Cl2N
IUPAC Name:
2,3-dichloroaniline
Details on test material:
- Name of test material (as cited in study report): 2,3-dichloroaniline
- Analytical purity: not reported (technically pure)

Test animals

Species:
rat
Strain:
Wistar
Sex:
male/female
Details on test animals or test system and environmental conditions:
male + female Wister rats, weight 160-180 g, husbandry: standardised conditions, cage (makrolon type III)

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
polyethylene glycol
Details on oral exposure:
2,3-dichloroaniline was emulsified in lutrol (polyethylenglycol 400), animals received 0.5 ml of the emulsion per 100 g bw
Doses:
m: 250, 500, 1000, 1500, 2000, 2500, 2600, 3000, 3500, 5000 mg/kg bw;
f: 500, 1000, 1500, 2000, 2500,3000, 3250, 3500 mg/kg bw
No. of animals per sex per dose:
15
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
2 635 mg/kg bw
Sex:
female
Dose descriptor:
LD50
Effect level:
2 489 mg/kg bw

Any other information on results incl. tables

Clinical signs:
reduced general condition,difficulties in breathing;
rats with higher dosages revealed additionally tremor, cyanotic  discoloration of skin and visible mucous membranes:

dosages in mg/kg bw: number of dead rats/number of rats with clinical  signs/number of rats used/time of death:
       Males:                //    Females:
0250:  0 /  0 / 15 / --......//    ---------------
0500:  1 / 15 / 15 / 5d......//    0 /  0 / 15 / --
1000:  0 / 15 / 15 / --......//    0 / 15 / 15 / --
1500:  0 / 15 / 15 / --......//    1 / 15 / 15 / 2h
2000:  3 / 15 / 15 / 1d......//    6 / 30 / 30 / 1h-2d
2500:  4 / 15 7 15 / 18'-1d..//    5 / 15 / 15 / 2h-3d
2600:  8 / 15 / 15 / 1h-1d...//....---------------
3000: 10 / 15 / 15 / 1d......//   10 / 15 / 15 / 2h-1d
3250: ---------------------..//   14 / 15 / 15 / 1h-1d
3500: 14 / 15 / 15 / 2h-1d...//   15 / 15 / 15 / 3h-2d
5000: 15 / 15 / 15 / 1h-1d...//   --------------------

calculation:
LD50 (male):  2635 mg/kg bw
LD50 (female):  2489 mg/kg bw

Applicant's summary and conclusion

Executive summary:

Bayer, 1976:


The LD50 in male rats is 2635 mg/kg bw for female rats the LD50 is 2489 mg/kg bw.