Registration Dossier
Registration Dossier
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EC number: 210-157-9 | CAS number: 608-27-5
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- from 7-7-1988 to 15-7-1988
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,3-dichloroaniline
- EC Number:
- 210-157-9
- EC Name:
- 2,3-dichloroaniline
- Cas Number:
- 608-27-5
- Molecular formula:
- C6H5Cl2N
- IUPAC Name:
- 2,3-dichloroaniline
- Details on test material:
- - Name of test material (as cited in study report): 2,3-dichloroaniline
- Physical state: solid (dark brown)
- Analytical purity: 99.1%
- Purity test date: 15-4-1988
- Lot/batch No.: 3
- Storage condition of test material: refrigerator
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella typhimurium TA1535, TA100, TA1537, TA 98
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- 0, 20 - 12500 µg/plate dissolved in DMSO
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-nitro-1,2-phenylene-diamine; 2-aminoanthracene; nitrofurantoin
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 300 µg/plate and above
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Executive summary:
Bayer, 1988:
The AMES test for 2,3-DCA showed no potential for mutagenicity in all 4 strains that were tested.
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