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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vivo mammalian cell study: DNA damage and/or repair
Remarks:
Type of genotoxicity: DNA damage and/or repair
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1999
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No guideline study, well documented

Data source

Reference
Reference Type:
publication
Title:
Induction of unscheduled DNA synthesis in hairless mouse epidermis by skin carcinogens
Author:
Mori et al
Year:
1999
Bibliographic source:
J Tox Sci; Vol 24, 3, p.217-226

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Skin epidermis instead of liver cells were investigated for unscheduled DNA synthesis after topical application of the test item to mouse skin.
GLP compliance:
no
Type of assay:
unscheduled DNA synthesis

Test material

Constituent 1
Reference substance name:
Decyloxirane
EC Number:
220-667-3
EC Name:
Decyloxirane
Cas Number:
2855-19-8
IUPAC Name:
2-decyloxirane

Test animals

Species:
mouse
Strain:
other: HOS-HR1
Sex:
female
Details on test animals or test system and environmental conditions:
Food and water ad libitum. 12 h dark/light cycle.

Administration / exposure

Route of administration:
dermal
Vehicle:
acteone
Details on exposure:
before applying the test substance, the skin was stripped with a cellulose tape.
Duration of treatment / exposure:
2 h
Frequency of treatment:
once
Post exposure period:
none
Doses / concentrationsopen allclose all
Remarks:
Doses / Concentrations:
topical application
Basis:
nominal conc.
0.5%
Remarks:
Doses / Concentrations:
topical application
Basis:
nominal conc.
1%
Remarks:
Doses / Concentrations:
topical application
Basis:
nominal conc.
2%
No. of animals per sex per dose:
5
Control animals:
yes, concurrent vehicle
Positive control(s):
yes

Examinations

Tissues and cell types examined:
skin epidermis
Evaluation criteria:
Ratio between DNS synthesis in presence and absence of hydroxy urea.

Results and discussion

Test results
Sex:
female
Genotoxicity:
negative
Toxicity:
not examined
Vehicle controls validity:
valid
Negative controls validity:
not examined
Positive controls validity:
valid

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative
In this test system no unscheduled DNA synthesis was induced with the test item.
Executive summary:

In an adopted UDS assay, five mice received a single topical dose (0, 0.5, 1, 2%) of the test item. The skin epidermis was extracted and the unscheduled DNA synthesis was assessed using (3H)dTHd as marker. and a liquid scintillation counter as detector. No DNA synthesis was induced by the test item. The positive controls (DMBA, MNNG, BaP) were valid..