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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The 96 h LC0 value of the test substance (including inorganic components) was determined to be ≥84.3 mg/L 

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
84.3 mg/L

Additional information

A study was performed to assess the acute toxicity of test substance to Zebra fish (Brachydanio rerio) under static conditions. The study was conducted in accordance with EU Method C.1. and OECD Guideline 203. 

Groups of ten fish of the recommended size were exposed to a limit test concentration of nominally 100 mg/L of test substance dissolved in water. Analysis was performed for the test concentration plus a control on daily basis. All results are expressed in terms of mean measured concentrations.Measured concentrations correspond to 85.9, 85.9, 81.8, 81.8 and 85.9% of nominal values at 0, 24, 4, 72 and 96 h respectively. The hardness of the dilution water used was 11.6 °dH (=207.1 mg/L CaCO3).

Observations were made on the number of dead fish and the incidence of sub-lethal effects after 2, 24, 48, 72 and 96 h of exposure. 

All results are expressed in terms of mean measured concentrations. Measured concentrations correspond to 85.9, 85.9, 81.8, 81.8 and 85.9% of nominal values at 0, 24, 4, 72 and 96 h respectively. The hardness of the dilution water used was 11.6 °dH (=207.1 mg/L CaCO3).

The 96 h LC0 value of the test substance (including inorganic components) was determined to be ≥84.3 mg/L (Dr. Bruns, 2002).