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Environmental fate & pathways

Biodegradation in soil

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Administrative data

Endpoint:
biodegradation in soil
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Paper presents description of test method and results of degradation step by nitrification. However, not adequate for the purpose of hazard/risk assessment or hazard classification.

Data source

Reference
Reference Type:
publication
Title:
Biodegradation of aromatic ethers by nitrifying bacteria
Author:
Chang, S.W.
Year:
1999
Bibliographic source:
International in situ and on-site bioremediation symposium, 5th, San Diego (7), pp. 131-135

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Cells from a culture of the soil nitrifying bacterium Nitrosomonas europaea were tested for their capability to degrade the test substance in a batch experiment. Die-away of parent and formation of oxidation products in time was detected by HPLC
GLP compliance:
not specified
Test type:
laboratory

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: liquid
Details on test material:
no data
Radiolabelling:
no

Study design

Oxygen conditions:
aerobic
Soil classification:
other: not tested in soil; Cell culture of one species
Details on soil characteristics:
not applicable
Parameter followed for biodegradation estimation:
other: analysis of test material and degradation products
Details on experimental conditions:
Nitrosomonas europaea culture in phosphate buffer, 30 degree C

Results and discussion

Transformation products:
yes
Identity of transformation productsopen allclose all
No.:
#1
Reference
Reference substance name:
Unnamed
Inventory number:
InventoryMultipleMappingImpl [inventoryEntryValue=EC 201-964-7]
IUPAC name:
2-methoxyphenol
CAS number:
90-05-1
No.:
#2
Reference
Reference substance name:
Unnamed
IUPAC name:
catechol
Identifier:
common name
Identity:
catechol
Details on transformation products:
no further details
Evaporation of parent compound:
not specified
Volatile metabolites:
not specified
Residues:
not specified
Details on results:
In general, degradation rates decrease when the number of methoxyl groups on a benzene molecule increase. Therefore veratrole degrades slower than anisole.
Results with reference substance:
no data

Any other information on results incl. tables

Reaction is catalyzed by the non-specific ammonia-oxidizing enzyme ammonia monooxygenase.

Applicant's summary and conclusion

Executive summary:

The test substance is degraded by Nitrosomonas europaea. Transformation products are 2 -methoxyphenol and catechol. The process is catalysed by the presence of the non-specific enzyme ammonia mono-oxygenase (AMO).

The test is performed with a culture of one specific bacterium and does not represent a system or data that can be used to derive degradation rates relevant for the environment. Thus the study is not adequate for hazard/risk assessment or classification.