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Diss Factsheets
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EC number: 200-939-8 | CAS number: 76-16-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP compliant, near guideline study, available as unpublished report, fully adequate for assessment
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Genotoxicity testing of the Halon replacement candidates trifluoroiodomethane (CF3I) and 1,1,1,2,3,3,3-heptafluoropropane(HFC227ea) using the Salmonella typhimurium and L5178Y mouse lymphoma mutation assays and the mouse micronucleus test.
- Author:
- Dodd DE, Ledbetter AD, Mitchell AD
- Year:
- 1 997
- Bibliographic source:
- Inhalation Toxicology 9(2):111-31
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- GLP compliance:
- yes
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- 1,1,1,2,3,3,3-heptafluoropropane
- EC Number:
- 207-079-2
- EC Name:
- 1,1,1,2,3,3,3-heptafluoropropane
- Cas Number:
- 431-89-0
- Molecular formula:
- C3HF7
- IUPAC Name:
- 1,1,1,2,3,3,3-heptafluoropropane
- Details on test material:
- The test material, a colorless gas, was received in a steel gas container from ManTech/Dayton on April 7, 1994, then transferred to Allen Ledbetter, ManTech/RTP, who was respinsible for handling, storage, and dilution of the test material. The HFC-227ea was stored at ManTech/RTP at room temperature (approximately 72°F). ManTechiDayton documented the strength, purity, and composition of the test material and provided a Material Safety and Data Sheet (MSDS) from Great Lakes Chemical Corporation for HFC-227ea.
1
Method
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Metabolic activation system:
- rat liver S9 homogenate
- Test concentrations with justification for top dose:
- Nominal concentrations:
Preliminary assay: 100000; 200000; 300000; 400000; 500000; 600000; 700000; 800000; 900000 ppm
Mutagenesis assay: 100000; 300000; 500000; 700000; 900000 ppm
IR determined concentrations:
Preliminary assay: 63,967, 147,112; 204,380, 269,853, 344,792; 378,811; 423,733; 458,673; 538,535 ppm
Mutagenesis assay: 84,765; 274,259; 408,759; 518,570; 568,484 ppm
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- no
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: hycanthone and cyclophosphamide
Results and discussion
Test results
- Key result
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- It was concluded that the substance did not demonstrate mutagenic potential in this in vitro cell mutation assay, under the experimental conditions described.
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