Alcohols, C20-22, reaction products with phosphorus oxide (P2O5)

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2008-10-27 to 2008-11-06

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Deviations:

not applicable

Principles of method if other than guideline:

not concerned

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 70-75°C
- Boiling point: >300°C
- Vapour pressure: 6.66 x 10-5 Pa.
- Water solubility (under test conditions): <1mg/L



OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: not considered as ready biodegradable

Analytical monitoring:

no

Vehicle:

yes

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Common name: Pseudokirchnerella subcapitata (reported as Raphidocelis subcapitata)
- Strain: CCAP 278, 4
- Method of cultivation: culltivated in freswater in darkness


ACCLIMATION
- Acclimation period: 4 days
- Culturing media and conditions (same as test or not):yes
- Any deformed or abnormal cells observed: after the 4 days of acclimatation the cells are observed under microscop (meaured and morphology analysing)

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

After each 24h period the concentration of Algae was controled by particle counter

Test temperature:

21-25°C +- 20°C

pH:

between 6.9 and 7.9

Nominal and measured concentrations:

1; 10 ; 100mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: lfask erlenmeyer (250ml)
- Initial cells density: 10000 algues at the start of the study
- No. of organisms per vessel:10000 per erlenmeyer
- No. of vessels per concentration (replicates):3
- No. of vessels per control (replicates):6
-
GROWTH MEDIUM
- Standard medium used: yes

OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH:no
- Photoperiod:continuous
- Light intensity and quality:4440 lux -8880


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter


- Range finding study
- Test concentrations: 1; 10 ; 100 mg/L
- Results used to determine the conditions for the definitive study:OEDCD guideline 23

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Results with reference substance (positive control):

the latest test (june 2008) gives an result of EC50(72h)=0.69mg/L

Reported statistics and error estimates:

LOD =0.015mg/L
LOQ=0.05mg/L

Toxicity of the test item LCE07105 was performed on the freshwater unicellular green algae "Pseudokirchneriella subcapitata" according to the OECDE 201 guideline (screening test). Results obtained show a slight toxicity of the test item with an inhibition of the average specific growth rate in comparison to the control 50% at the highest concentration of 100mg/L. For the reason, the EC50(0-72h) is superior to the highest tested concentration. In order to control and confirm the test item solubilisation and its stability throughout the study, an analysis of the test solution was performed at the 100mg/L test item concentration and in the filtered control: Total phosphorus was chosen as tracer and was analyzed at the start and the end of the study.

Nominal test item concentration in mg/L	total [P]
	at 0h	at 72h
filtered control
100mg/L	detected 0.056mg/L(indicative value: 0;046mg/L)	detected 0.056mg/L(indicative value: 0;046mg/L)

Validity criteria fulfilled:

yes

Conclusions:

EC50(72h)>100mg/L in a limit test condition

Executive summary:

Toxicity of the test item LCE07105 was performed on the freshwater unicellular green algae "Pseudokirchneriella subcapitata" according to the OECDE 201 guideline (screening test). Results obtained show a slight toxicity of the test item with an inhibition of the average specific growth rate in comparison to the control <50% at the highest concentration of 100mg/L. For the reason, the EC50(0-72h) is superior to the highest tested concentration. In order to control and confirm the test item solubilisation and its stability throughout the study, an analysis of the test solution was performed at the 100mg/L test item concentration and in the filtered control: Total phosphorus was chosen as tracer and was analyzed at the start and the end of the study.

Nominal test item concentration in mg/L	total [P]
	at 0h	at 72h
filtered control	<LOD	<LOD
100mg/L	detected <0.056mg/L(indicative value: 0;046mg/L)	detected 0.056mg/L(indicative value: 0;046mg/L)

Description of key information

Toxicity of the registered substance was performed on the freshwater unicellular green algae "Pseudokirchneriella subcapitata" according to the OECDE 201 guideline (screening test). The EC50(0-72h) is superior to the highest tested concentration (100 mg/L).

 

Key value for chemical safety assessment

EC50 for freshwater algae:

100 mg/L