Alcohols, C20-22, reaction products with phosphorus oxide (P2O5)

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 2008

Reliability:

1 (reliable without restriction)

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: 70-75°C
- Boiling point: >300°C
- Vapour pressure: 6.66 x 10-5 Pa.
- Water solubility (under test conditions): <1mg/L



OTHER PROPERTIES (if relevant for this endpoint)
- Results of test for ready biodegradability: not considered as ready biodegradable

Analytical monitoring:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name:Daphnia magna
- Age at study initiation (mean and range, SD):24 Hours
- Length at study initiation (length definition, mean, range and SD): between 560µm to 800µm

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

CaCO3: 250 mg/L

Test temperature:

20+-1°C

pH:

7.9

Dissolved oxygen:

8.2 to 8.5

Salinity:

CaCl2 H2O=297mg/L
MgCl26H2O=167 mg/L
NaHCO3=200mg/L
K2SO4=26mg/L

Nominal and measured concentrations:

1; 10; 100 mg/L

Details on test conditions:

TEST SYSTEM

- Renewal rate of test solution (frequency/flow rate):every 24 hours
- No. of organisms per vessel:5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):4



TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Details on results:

no sign of stress was observed on the daphnids after the 48 hours of exposition

Results with reference substance (positive control):

the last toxic response obtained on the reference item potassium dichromate (6th October 2008) gives an EC50-24 hours of 1.09 mg/L, indicating that the daphnids sensitivity is correct (within the accepted interval of 0.6-2.1 mg/L fixed by the international standard ISO 6341 may 1996) and in accordance with the historical data obtained by the laboratory.

Reported statistics and error estimates:

none

The acute immobilisation screening test of the micro crustacean Daphnia magna was performed on the test item "LCE07105". The results obtained on the toxicological endpoint "immobilisation" gives an EC50 -48 hours superiot to the highest tested concentration, i. e 100mg/L.

This result espressed in mg/L of the nominal concentration shows that no toxicity was observed in the concentrations selected in this test on the micro crustacean Daphnia magna. No sign of stress was observed on the Daphnids during 48 hours of the experiment.

In order to control and to confirm the test item solubilisation and its stability throughout the study, an analysis of the test solution contained in the aquarium was performed at the 100mg/L test item concentration and in the filtered control: Total phosphorus was chosen as tracer and was analyzed at the start and the end of the study (as the test was performed with semi static condition, the final sample was aged of 24 hours instead of 48 hours).

Results are presented as follow:

Nominal test Item concentrations in mg/L	totol [P]
	at 0h	at 48h
Filtered control
100	0.049 mg/L	0.056mg/L

Validity criteria fulfilled:

yes

Conclusions:

EC50-48 hours of the test item on Cladocera micro crustacean Daphia magna is superior to the highest tested concentration, i.e 100mg/L, value expressed in nominal concentration.

Executive summary:

The acute immobilisation screening test of the micro crustacean Daphnia magna was performed on the test item "LCE07105". The results obtained on the toxicological endpoint "immobilisation" gives an EC50 -48 hours superiot to the highest tested concentration, i. e 100mg/L.

This result espressed in mg/L of the nominal concentration shows that no toxicity was observed in the concentrations selected in this test on the micro crustacean Daphnia magna. No sign of stress was observed on the Daphnids during 48 hours of the experiment.

In order to control and to confirm the test item solubilisation and its stability throughout the study, an analysis of the test solution contained in the aquarium was performed at the 100mg/L test item concentration and in the filtered control: Total phosphorus was chosen as tracer and was analyzed at the start and the end of the study (as the test was performed with semi static condition, the final sample was aged of 24 hours instead of 48 hours).

Results are presented as follow:

Nominal test Item concentrations in mg/L	totol [P]
	at 0h	at 48h
Filtered control	<LOD	<LOD
100	0.049 mg/L	0.056mg/L

Description of key information

The acute immobilisation screening test of the micro crustacean Daphnia magna was performed on the registered substance. The results obtained on the toxicological endpoint "immobilisation" gives an EC50 -48 hours superiot to the highest tested concentration, i. e 100mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L