Alcohols, C20-22, reaction products with phosphorus oxide (P2O5)

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 12 February 2008 to 07 August 2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

OECD Guideline No. 105 (1995)

Deviations:

yes

Remarks:

The sand was washed only one time with acetone instead of two times as described in the corresponding procedure.The pH of the collected fraction after the column elution was not taken. Not considered to have affected the results quality/ interpretation.

GLP compliance:

yes (incl. QA statement)

Type of method:

column elution method

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Batch No. LCE07101

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test item was stored in darkness at room temperature.

Key result

Water solubility:

<= 1 mg/L

Conc. based on:

test mat.

Loading of aqueous phase:

10 mg/L

Incubation duration:

ca. 25 h

Temp.:

20 °C

Remarks on result:

other: The pH was not measured (see deviation)

Details on results:

The PH of the collected fraction after the column elution was not taken.This deviation was not considered to have affacted the quality or the interpretaion of the results obtained .

Preliminary test:

In the first step it was shown after additional volumes of water that about 100 mg of the test item were insoluble in 10mL of water.

In the second step it was shown that:

-About 10 mg of the test item were insoluble in 100 mL of water,

-About 2.3 mg of the test item were insoluble in 230 mL of water,

after 24h at 20°C and magnetically stirrring.

The solubility in water of the test item is lower than 10 mg/L.Henceforth the column elution method was chosen for the determination of the hydrosolubility.

Main test:

The results of the hydrosolubility determination are obtained after quantification of the test item by gas chromatography using a flame ionization detector.

Flow rate at 12.5 mL/hours

Equilibration time : 25 hours

Test solution after	Alcohols concentration  (mg/L)
25 h	< LOQ
26 h	< LOQ
27 h	< LOQ
28 h	< LOQ
29 h	< LOQ
Mean Value	< LOQ

LOQ : Limit Of Quantification (0.5 mg/L)

Flow rate at 25 mL/hour

Equilibration time : 25 hours

Test solution after	Alcohols concentration (mg/L)
25 h	< LOQ
26 h	< LOQ
27 h	< LOQ
28 h	< LOQ
29 h	< LOQ
Mean Value	< LOQ

LOQ : Limit Of Quantification (0.5 mg /L)

The alcohols (C20 and C22), the main substances of the test item were ananlysed as silyl derivatives by gas chromatography using a FID detector.The limit of quantification of the alcohols was 0.5 mg/L.

The analysis of the alcohols in the fractions obtained at elution flow rates of 12.5 and 25 mL/h showes tha the hydrosolubility of the alcohols is lower than 0.5 mg/L.

Conclusions:

Interpretation of results : slightly soluble (0.1-100 mg/L)

The hydrosolubility of the test item is equal or lower than 1 mg/L.

Executive summary:

As the preliminary test showed a hydrosolubility lower than 10mg/L, the column elution method was taken to determine the hydrosoolubility of the test substance. The alcohols (C20 and C22), the main substances of the test item were analysed as silyl derivates by gas chromatography using a FID detector. The limit of auqntification of the alcohols was 0.5 mg/L.

The analysis of the alcohols int he fractions obtained at elution flow rates of 12.5 and 25mL/h showed that the hydrosolubility of the alcohols is lower than 0.5 mg/L. The hydrosolubility of the test item is equal or lower than 1 mg/L.

Description of key information

In compliance with Commission Directive 92/69/EEC - A6 method (1992) OECD Guideline No. 105 (1995) a solubility test was performed to assess the hydrosolubility of the test item.

As the preliminary test showed a hydrosolubility lower than 10 mg/L, the column elution method was taken to determine the hydrosolubility of the test item.

The alcohols (C20 and C22), the main substances of the test item were analysed as silyl

derivatives by gas chromatography using a FID detector. The limit of quantification of the alcohols was 0.5 mg/L.

The analysis of the alcohols in the fractions obtained at elution flow rates of 12.5 and

25 mL/h showed that the hydrosolubility of the alcohols is lower than 0.5 mg/L.

The hydrosolubility of the test item was determined to be less than or equal to 1 mg/L.

Key value for chemical safety assessment

Water solubility:

1 mg/L

at the temperature of:

20 °C