Poly(oxy-1,2-ethanediyl), α-(carboxymethyl)-ω-hydroxy-, C16-18 (even numbered) and C18-unsatd. alkyl ethers

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1998-02-23 to 1998-03-20

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

((adopted 17-07-1992)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

(adopted 31-07-1992)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Remarks:

TOC analysis

Details on sampling:

- Concentrations: 5, 10, 22, 50 mg/L

Vehicle:

no

Details on test solutions:

24 h stirring

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zenbra fish
- Strain:Hamilton-Buchanan
- Source: own breed HMR Deutschland GmbH, Global Preclinical Development - Drug Safety
- Length at study initiation (length definition, mean, range and SD): 2.8 cm (2.6 - 3.0 cm / s = 0.13)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

21.1 - 21.8 °C

pH:

7.1 - 8.2

Dissolved oxygen:

5.9 - 9.8 mg/L

basin of 10 mg/L group was ventilated after 72 h
basin of 22 mg/L group was ventilated after 48 h

Nominal and measured concentrations:

nominal: 0, 5, 10, 22, 50 mg/L
mean measured: 0, 2.36, 7.19, 21.75, 69.02 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: no data
- Aeration: yes (10 mg/L group was aerated starting at 72 h, 22 mg/L group was aerated starting at 48 h with a bubble frequence of 1-3 /s)
- No. of organisms per vessel: no data
- No. of vessels per concentration (replicates): no data
- No. of vessels per control (replicates): no data


TEST MEDIUM / WATER PARAMETERS
- no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mortality, abnormal behaviour atfer 24, 48, 72, 96 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - 2.27

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

9.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% c.i. 5.5 - 14.3 mg/L

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

50 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- at 5 mg/L no mortality or sublethal effects were noted, at 10, 22 and 50 mg/L mortality and sublethal effects were observed
- Mortality of control: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:

Reported statistics and error estimates:

Probit analysis

Validity criteria fulfilled:

not specified

Conclusions:

The 96 LC50 of Polyoxyethylene(1 to 2.5) oleyl ether carboxylic acid was 9.1 mg/L (95% c.i. 5.5 - 14.3 mg/L) based on nominal concentrations.

Executive summary:

In a 96-h acute toxicity study according to OECD Guideline 203 (1992) and EU method C.1 (1992), zebrafish (Danio rerio) were exposed to Polyoxyethylene(1 to 2.5) oleyl ether carboxylic acid at nominal concentrations of 0 (control), 0, 5, 10, 22, 50 mg/L under static conditions. Mean measured concentrations (TOC analysis) were 0, 2.36, 7.19, 21.75, 69.02 mg/L. Turbidity was observed in all test solutions, and measured concentrations differed 47.2 - 138% from nominal. Thus, effect concentrations are given as nominal.

The 96 LC50 of Polyoxyethylene(1 to 2.5) oleyl ether carboxylic acid was 9.1 mg/L (95% c.i. 5.5 - 14.3 mg/L) based on nominal concentrations.

At 5 mg/L no mortality or sublethal effects were noted, at 10, 22 and 50 mg/L mortality and sublethal effects were observed

This toxicity study is classified as acceptable and satisfies the guideline requirement for an acute toxicity study with zebrafish (Danio rerio).

 

Results Synopsis

Test Organism Size/Age: Danio rerio

Test Type: Static

96 h LC50: 9.1 mg/L (95% c.l. 5.5 - 14.3 mg/L), nominal

Endpoint(s) Effected: Mortality 

Description of key information

96 LC50 = 9.1 mg/L (95% c.i. 5.5 - 14.3 mg/L), nominal (Danio rerio) (RL2, GLP; OECD TG 203/EU method C.1)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

9.1 mg/L

Additional information

In a 96-h acute toxicity study according to OECD Guideline 203 (1992) and EU method C.1 (1992), zebrafish (Danio rerio) were exposed to Polyoxyethylene(1 to 2.5) oleyl ether carboxylic acid at nominal concentrations of 0 (control), 5, 10, 22, 50 mg/L under static conditions. Mean measured concentrations (TOC analysis) were 0, 2.36, 7.19, 21.75, 69.02 mg/L. Turbidity was observed in all test solutions, and measured concentrations differed 47.2 - 138% from nominal. Thus, effect concentrations are given as nominal.

The 96 LC50 of Polyoxyethylene(1 to 2.5) oleyl ether carboxylic acid was 9.1 mg/L (95% c.i. 5.5 - 14.3 mg/L) based on nominal concentrations.

At 5 mg/L no mortality or sublethal effects were noted, at 10, 22 and 50 mg/L mortality and sublethal effects were observed