Reaction mass of Cobaltate(1-), bis[6-(amino-κN)-5-[2-[2-(hydroxy-κO)-4-nitrophenyl]diazenyl-κN1]-N-methyl-2-naphthalenesulfonamidato(2-)]-, sodium (1:1) and disodium [6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]-N-methylnaphthalene-2-sulphonamidato(2-)][6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]naphthalene-2-sulphonato(3-)]cobaltate(2-)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1991

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

Principles of method if other than guideline:

Method A-11

GLP compliance:

no

Specific details on test material used for the study:

None

Analytical monitoring:

yes

Details on sampling:

Not available

Vehicle:

yes

Details on test solutions:

Not available

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Zebrafish:(Danio rerio)
Replicates: 10 per test concentration
Age: about 4-6 months
Size: about 3 cm
Weight: 0.25-0.35 g
Supplier: West Aquarium GmbH, D-3422 Bad Lauterberg

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not available

Hardness:

Not available

Test temperature:

ca. 23 °C

pH:

8.0 - 8.1

Dissolved oxygen:

82, 94 and 87 %

Salinity:

Not available

Conductivity:

Not available

Nominal and measured concentrations:

100, 300 and 1000 mg/l

Details on test conditions:

Not available

Reference substance (positive control):

not specified

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

449.95 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

305.77 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

At 100 mg/l: all fish were alive after 96 h
At 300 mg/l : 7 fish were alive after 96 h
At 1000 mg/l : all fish died after 48 h

Results with reference substance (positive control):

Not available

Reported statistics and error estimates:

Not available

Sublethal observations / clinical signs:

Concentration	Survivors
	1 h	4 h	24 h	48 h	72 h	96 h
100 mg/l	10	10	10	10	10	10
300 mg/l	10	10	9	9	7	7
1000 mg/l	10	10	1	0	0	0

Validity criteria fulfilled:

no

Conclusions:

The LC50 of FAT 20036/E in a short term fish toxicity test was found to be 305.77 mg/l after 96h of exposure.

Executive summary:

A test was carried out in Zebra fish to determine short term toxicity of FAT 20036/E according to method equivalent or similar to OECD Guideline 203 (Fish, Acute Toxicity Test) at concentrations of 100, 300 and 1000 mg/l.

At 100 mg/l: all fish were alive after 96 h

At 300 mg/l : 7 fish were alive after 96 h

At 1000 mg/l : all fish died after 48 h

The following results were found:

LC0 (48h): 100 mg/L

LC50 (48h): 449.95 mg/L

LC100 (48h): 1000 mg/L

LC0 (96h): 100 mg/l

LC50 (96h): 305.77 mg/l

LC100 (96h): 1000 mg/l

The LC50 of FAT 20036/E in a short term fish toxicity test was found to be 305.77 mg/l after 96h of exposure.

Description of key information

The LC50 for FAT 20036 in the acute fish toxicity test was determined to be 449.95 mg/l at 48 hours exposure time and 305.77 mg/l at 96 hours of exposure time.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

305.77 mg/L

Additional information

A key study was carried out in Zebra fish to determine short term toxicity of FAT 20036/E equivalent or similar to OECD Guideline 203 (Fish, Acute Toxicity Test) at concentrations of 100, 300 and 1000 mg/l.

The LC50 in the acute fish toxicity test was determined to be 449.95 mg/l at 48 hours exposure time and 305.77 mg/l at 96 hours of exposure time.

In several supporting studies, following results were obtained:

TL0 (48 h) – 20, 100 and 30 mg/l

TL50 (48 h) – 45, 586, 103 and 274 mg/l

TL100 (48 h) -100 and 1000 mg/l

TL0 (96 h) – 100 and <30 mg/l

TL50 (96 h) – 382, 42 and 183 mg/l

TL100 (96 h) – 1000 and 300 mg/l