Reaction mass of Cobaltate(1-), bis[6-(amino-κN)-5-[2-[2-(hydroxy-κO)-4-nitrophenyl]diazenyl-κN1]-N-methyl-2-naphthalenesulfonamidato(2-)]-, sodium (1:1) and disodium [6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]-N-methylnaphthalene-2-sulphonamidato(2-)][6-amino-5-[(2-hydroxy-4-nitrophenyl)azo]naphthalene-2-sulphonato(3-)]cobaltate(2-)

Endpoint summary

Description of key information

Short-term toxicity tests are available for all three trophic levels (fish, invertebrates and lemna). The 96 -hour LC50 in zebrafish is 305.77mg/L, the 24 -hour EC50 in Daphnia magna is >131 mg/L, and the 7 d ErC50 and EyC50 in lemna is 28.31 mg/L and 14.68 mg/l respectively . In an activated sludge respiration inhibition test a 3-hour IC50 value of >320 mg/L was observed.

Additional information

A key study was carried out in Zebra fish to determine short term toxicity of FAT 20036/E equivalent or similar to OECD Guideline 203 (Fish, Acute Toxicity Test) at concentrations of 100, 300 and 1000 mg/l. The LC50 in the acute fish toxicity key test was determined to be 449.95 mg/l at 48 hours exposure time and 305.77 mg/l at 96 hours of exposure time.

A key study was conducted for FAT 20036/F to evaluate its acute toxicity to Daphnia magna. The tests were run according to the OECD Guideline 202 and in accordance with GLP. According to the results of the test, the NOEC and EC0 value after 48 hours were determined to be 131 mg/l, the EC50 and the EC 100 value could not be calculated, but are clearly higher than 131 mg/l

In a key study, Lemna gibba (Duckweed) were exposed to the test item (FAT 20036/G TE) at different test concentrations viz.,0.3, 0.87, 2.52, 7.32, 21.22 and 61.53 mg/L. Initially on day '0', 12 fronds were inoculated in beaker containing 200 ml of test medium (20X-AAP medium). Three replicates for each test concentration and six replicates for control were maintained. The test item was formulated in 20X-AAP medium. Prior to exposure, Lemna gibba was pre-cultured in the 20X-AAP medium for period of '8' days and the pre-culture was found healthy. After exposure, Lemna gibba was observed for frond count on days '3', '5' and '7'. Dry weight of the fronds were recorded at start (pre-culture representative samples) and at the end of the test. Fronds were observed for their appearance on days '3', '5' and '7. All fronds were found normal and healthy in all replicates of control and test concentrations except 21. 22 and 61.53 mg/L where necrosis observed in fronds exposed to 61.53 mg/L concentration on day '5' and in addition to necrosis, root filaments were turned blue in 21.22 and 61.53 concentrations on day '7'. The doubling time (T_d) of frond number in the control group was calculated as 1.62 day.

Frond Numbers: From the average specific growth rate recorded with the different test concentrations viz.,0.3, 0.87, 2.52, 7.32, 21.22 and 61.53 mg/L, the test concentrations that inhibited 50% growth (E_rC50) was calculated as 28.31 mg/L with upper confidence limits of 29.51 mg/L and lower confidence limits of 27.16 mg/L.The percent inhibition on the average specific growth rate on day '7' for the test concentrations of 0.3, 0.87, 2.52, 7.32, 21.22 and 61.53 mg/L was calculated as 0.02%, 0.02%, 5.94%, 7.34%, 31.37% and 90.46% respectively. From the yield recorded with the different test concentrations viz.,0.3, 0.87, 2.52, 7.32, 21.22 and 61.53 the concentration that inhibited 50% yield (E_yC₅₀) was calculated as 14.68 mg/L with upper confidence limits of 15.39 mg/L and lower confidence limits of 14.01 mg/L.

The percent inhibition on the yield on day '7' for the test concentrations of 0.3, 0.87, 2.52, 7.32, 21.22 and 61.53 mg/L was calculated as 0.07%, 0.07%, 17.19%, 20.81%, 64.18% and 98.26% respectively. The no observed effect concentration (NOEC) was calculated as 0.87 mg/L and the low observed effect concentration (LOEC) was calculated as 2.52 mg/L.

Frond Dry Weight: From the average specific growth rate recorded with the test concentrationsviz.,0.3, 0.87, 2.52, 7.32, 21.22 and 61.53 mg/L the concentration that inhibited 50% growth (E,C₅₀) was calculated as 24.22 mg/L with upper confidence limits of 25.24 mg/L and lower confidence limits of 23.24 mg/L. The percent inhibition on the average growth rate on day '7' for the concentration of 0.3, 0.87, 2.52, 7.32, 21.22 and 61.53 mg/L was calculated as 0.21%, 0.21%, 4.93%, 13.06%, 34.48 and 96.15% respectively. From the yield recorded with the test concentrations viz., 0.3, 0.87, 2.52, 7.32, 21.22 and 61.53 mg/L, the concentration that inhibited 50% yield (E_yC₅₀) was calculated as 11.93 mg/L with upper confidence limits of 12.51 mg/L and lower confidence limits of 11.38 mg/L. The percent inhibition on the yield on day '7' for the test concentrations of 0.3, 0.87, 2.52, 7.32, 21.22 and 61.53 mg/L was calculated as 0.29%, 0.29%, 15.34%, 35.83%, 70.17% and 99.48% respectively.

The no observed effect concentration (NOEC) was calculated as 0.87 mg/L and the low observed effect concentration (LOEC) was calculated as 2.52 mg/L.

A key study was performed to determine the toxicity of FAT 20036/F to activated sludge was determined in a 3-hours respiration inhibition test according to the EEC Method No. L 133/118 -122 and to the OECD Guideline No. 209. The test was performed in compliance with the Good Laboratory Practice (GLP) Regulations of Switzerland. The IC50 (3 h) of the test substance FAT 20036/F was found to be greater than 320.0 mg/l