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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
not specified
Principles of method if other than guideline:
Method A-11
GLP compliance:
no
Specific details on test material used for the study:
None
Analytical monitoring:
yes
Details on sampling:
Not available
Vehicle:
yes
Details on test solutions:
Not available
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Zebrafish:(Danio rerio)
Replicates: 10 per test concentration
Age: about 4-6 months
Size: about 3 cm
Weight: 0.25-0.35 g
Supplier: West Aquarium GmbH, D-3422 Bad Lauterberg
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
Not available
Hardness:
Not available
Test temperature:
ca. 23 °C
pH:
8.0 - 8.1
Dissolved oxygen:
82, 94 and 87 %
Salinity:
Not available
Conductivity:
Not available
Nominal and measured concentrations:
100, 300 and 1000 mg/l
Details on test conditions:
Not available
Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
449.95 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
305.77 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
At 100 mg/l: all fish were alive after 96 h
At 300 mg/l : 7 fish were alive after 96 h
At 1000 mg/l : all fish died after 48 h
Results with reference substance (positive control):
Not available
Reported statistics and error estimates:
Not available
Sublethal observations / clinical signs:

Concentration

Survivors

1 h

4 h

24 h

48 h

72 h

96 h

100 mg/l

10

10

10

10

10

10

300 mg/l

10

10

9

9

7

7

1000 mg/l

10

10

1

Validity criteria fulfilled:
no
Conclusions:
The LC50 of FAT 20036/E in a short term fish toxicity test was found to be 305.77 mg/l after 96h of exposure.
Executive summary:

A test was carried out in Zebra fish to determine short term toxicity of FAT 20036/E according to method equivalent or similar to OECD Guideline 203 (Fish, Acute Toxicity Test) at concentrations of 100, 300 and 1000 mg/l.

At 100 mg/l: all fish were alive after 96 h

At 300 mg/l : 7 fish were alive after 96 h

At 1000 mg/l : all fish died after 48 h

The following results were found:

LC0 (48h): 100 mg/L

LC50 (48h): 449.95 mg/L

LC100 (48h): 1000 mg/L

LC0 (96h): 100 mg/l

LC50 (96h): 305.77 mg/l

LC100 (96h): 1000 mg/l

The LC50 of FAT 20036/E in a short term fish toxicity test was found to be 305.77 mg/l after 96h of exposure.

Description of key information

The LC50 for FAT 20036 in the acute fish toxicity test was determined to be 449.95 mg/l at 48 hours exposure time and 305.77 mg/l at 96 hours of exposure time.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
305.77 mg/L

Additional information

A key study was carried out in Zebra fish to determine short term toxicity of FAT 20036/E equivalent or similar to OECD Guideline 203 (Fish, Acute Toxicity Test) at concentrations of 100, 300 and 1000 mg/l.

The LC50 in the acute fish toxicity test was determined to be 449.95 mg/l at 48 hours exposure time and 305.77 mg/l at 96 hours of exposure time.

In several supporting studies, following results were obtained:

TL0 (48 h) – 20, 100 and 30 mg/l

TL50 (48 h) – 45, 586, 103 and 274 mg/l

TL100 (48 h) -100 and 1000 mg/l

TL0 (96 h) – 100 and <30 mg/l

TL50 (96 h) – 382, 42 and 183 mg/l

TL100 (96 h) – 1000 and 300 mg/l