Chromium trioleate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: DIN 38412, part 15

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Chromium trioleate

EC Number:

244-256-3

EC Name:

Chromium trioleate

Cas Number:

21178-63-2

Molecular formula:

C18 H34 O2 .1/3 Cr

IUPAC Name:

chromium(3+) tris((9Z)-octadec-9-enoate)

Details on test material:

Chrom (III)-oleat
Test substance No.: 89/791
Batch No.: G 6/88
Purity: ca. 93 %

Solubility in water: < 0.8 mg/l

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was added to the test water without any pretreatment.

Test organisms

Test organisms (species):

Leuciscus idus

Details on test organisms:

TEST ORGANISM
- Common name: Golden orfe
- Source: Fischzucht Paul Eggers Hohenweststedt, FRG
- Length at study initiation: 9.2 cm (range: 8.2 - 10.6)
- Weight at study initiation: 8.1 g (range: 5.1 - 12.6)
- Diet: Growing feed F/B 50 SSNIFF Spezialdiaeten GMBH Soest, FRG (ad libitum)

ACCLIMATION
- Duration of housing and adaptation: About 6 months
- Acclimation conditions: Same as test
- Health during acclimation: 0%

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

Total hardness: 2.5 mmol/L

Test temperature:

21 - 22 °C

pH:

about 8.0

Nominal and measured concentrations:

Based on the results of a range finding study, the test concentrations, spaced by a factor of ca. 2.2, were fixed as follows: 0, 100, 215, 464, and 1000 mg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: All-Glass Aquarium (30 cm x 22 cm x24 cm)
- Aeration: Continuous aeration with oil-free air
- No. of organisms per vessel: 10
- Photoperiod: 16 hours light and 8 hours darkness

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

< 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 - 220 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

215 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Nominal Dead fish after
conc. 1h 4h 24h 48h 72h 96h
[mg/l]
-----------------------------------------------------------
100 0/10 0/10 1/10 1/10 1/10 1/10
215 0/10 0/10 10/10 10/10 10/10 10/10
464 0/10 10/10 10/10 10/10 10/10 10/10
1000 0/10 10/10 10/10 10/10 10/10 10/10

control 0/10 0/10 0/10 0/10 0/10 0/10
-----------------------------------------------------------

Intoxication symptoms:
----------------------
Apathy, narcotic-like state