Caramel (color)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2018

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Caramel (color)

EC Number:

232-435-9

EC Name:

Caramel (color)

Cas Number:

8028-89-5

Molecular formula:

C6H12OH

IUPAC Name:

Caramel

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Strauss clone 5, between 6 and 24 hours old and produced from parthenogenically reproducing brood female population were used. Daphnia are issued from the breeding lab. The selection of the juvenile was daily realized by filtration. The juvenile stemming at least from the second litter were used to start the test.

Study design

Test type:

static

Water media type:

other: deionised water

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

Between 140 and 250 mg CaCO3/L

Test temperature:

20°C +/- 2

pH:

pH: Between 6 and 9

Dissolved oxygen:

8.9 mg/L (in the lowest concentration producing the maximum effect at the end of the test)

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

0 and 100 mg/L

Details on test conditions:

Light/dark cycle: 16 h/8 h

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

The EC50 with reference item over a period of 24 hours was between 0,6 and 2,1 mg.L-1 (actual value: 1.43 mg.L-1).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The acute effects of Caramel Colour E150c diluted in OECD test medium at the definitive concentration of 100 mg.L-1 to Daphnia magna was investigated under laboratory conditions.
The study is valid since immobilization in the control was less than 10% (actual values: 0% in the definitive test), dissolved oxygen in the lowest concentration producing the maximum effect at the end of the test was ≥ 3 mg.L-1 (actual values: 8.9 mg.L-1 in the definitive test) and the EC50 with reference item over a period of 24 hours was between 0,6 and 2,1 mg.L-1 (actual value: 1.43 mg.L-1).
Based on the number of immobilized daphnids, the EC50 after a 24-hour and 48-hour exposure periods were greater than 100 mg.L-1.