N,N,4-trimethylpiperazine-1-ethylamine

Endpoint summary

Description of key information

Six ecotoxicological studies performed with the test substance are available. The acute toxicity study of rainbow trout (Envigo, 2018) resulted in an 96 hour LC50 > 29 mg/L (based on nominal concentrations). Based on this result it can be concluded that the test substance is not harmful to fish. Two relevant studies were identified and were used in a weight of evidence approach to assess acute toxicity to aquatic invertebrates in Daphnia magna according to OECD guideline 202. Based on nominal concentrations, the study of Migchielsen (2002) revelated a 48-h EC50 of 39 mg/L. An EC50 of 66 mg/L (based on nominal test concentrations) was found after an exposure of 48 hours in a static test in freshwater (Squance, 2018). The study of Migchielsen (2002) was considered for the PNEC derivation. Based on the results it can be concluded that the test substance is harmful to aquatic invertebrates. Two relevant studies were identified as key studies and were used in a weight of evidence approach to assess toxicity of the test substance to aquatic algae according to OECD guideline 201. Migchielsen (2002) obtained a nominal EC50 for growth rate inhibition (72-h ErC50) of 36 mg/L with a 95% confidence interval ranging from 30 to 43 mg/L. In the study of Envigo (2018), the 72-h NOEC and 72-h EC50 for growth rate were determined to be 3.2 mg/L and 29 mg/L respectively. The latter study was considered for the PNEC derivation. The substance can be considered as harmful to algae.

The study of Best (2017), investigating the toxicity of the test substance to microorganisms according to OECD guideline 209, was considered as the key study for endpoint coverage. The 3-h EC50 was >1000 mg/L. The 3-h NOEC was 1000 mg/L.