Potassium perchlorate

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: long-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Justification for type of information:

The studies were perfomred according to OECD guidelines without being conducted under GLP conditions. No detailed information on raw data are provided.

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))

Deviations:

not specified

GLP compliance:

no

Specific details on test material used for the study:

A 100 µg/mL certified perchlorate standard, granular sodium perchlorate and ammonium perchlorate were used to prepare the perchlorate solutions. All solutions were prepared in Milli-Q reverse osmosis water.

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Study type:

laboratory study

Remarks:

acute and long-term tests

Substrate type:

other: the test were conducted on filter, on sand or in artificial soil

Limit test:

no

Test temperature:

22 °C

Details on test conditions:

Mortality was the clinical endpoint examined in the 14-day acute toxicily tests. Mortality was recorded daily during each study. Earthwonns were classified as dead when they did not respond to a gentle mechanical stimulus at the anterior end.

Nominal and measured concentrations:

acute toxicity test, filter paper:
Nominal concentrations: 0, 0.1, 1, 700, 7000, 35000, 70000 µg/mL based on perchlorate anion

acute toxicity test, sand:
Nominal concentrations in sand: 0, 0.1, 1, 1000, 2000, 5000, 10000 µg/g based on perchlorate anion

reproductive toxicity test, artificial soil:
Nominal concentrations in soil: 0, 0.1, 10, 100, 1000 µg/g based on perchlorate anion

reproductive toxicity test, sand:
Nominal concentrations in sand: 0, 0.1, 1, 1000, 2000, 5000, 10000 µg/g based on perchlorate anion

Reference substance (positive control):

no

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

4 150 other: µg/mL

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

based on perchlorate anion

Basis for effect:

mortality

Remarks on result:

other: on filter paper

Key result

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

2 550 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

based on perchlorate anion

Basis for effect:

mortality

Remarks on result:

other: in sand

Key result

Duration:

28 d

Dose descriptor:

EC50

Effect conc.:

350 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

based on perchlorate anion

Basis for effect:

reproduction

Remarks:

cocoon production

Remarks on result:

other: in sand

Key result

Duration:

28 d

Dose descriptor:

EC50

Effect conc.:

1.3 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

based on perchlorate anion

Basis for effect:

reproduction

Remarks:

cocoon production

Remarks on result:

other:

Remarks:

in artificial soil

14 -day filter contact test with sodium perchlorate

perchlorate concentration (µg/mL)	number of earthworms survived/total number
0	5/5
0.1	3/5
1	2/5
700	4/5
7000	1/5
35,000	0/5
70,000	0/5

14 -day filter contact test with ammonium perchlorate

perchlorate concentration (µg/mL)	number of earthworms survived/total number
0	5/5
0.1	4/5
1	4/5
1000	5/5
10,000	0/5
50,000	0/5
100,000	0/5

14 -day test in sand with sodium perchlorate

perchlorate concentration (µg/mL)	number of earthworms survived/total number
0	10/10
0.1	10/10
1	10/10
1000	9/10
2000	7/10
5000	0/10
10,000	0/10

4 -week cocoon production test in artificial soil with sodium perchlorate

perchlorate concentration (µg/mL)	average cocooosn produced
0	2.0
0.1	1.25
10	2.0
100	0.75
1000	0

Validity criteria fulfilled:

not specified

Conclusions:

The LC50 value for Eisenia fetida according to this study can be stated to be 2550 mg/kg dw for an exposure time of 14 days in sand.
The EC50 value for Eisenia fetida according to this study can be stated to be 350 mg/kg dw for an exposure time of 28 days in sand and 1.3 mg/kg dw in artificail soil, respectively.

Description of key information

The LC50 value for Eisenia fetida according to this study can be stated to be 2550 mg/kg dw for an exposure time of 14 days in sand.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

2 550 mg/kg soil dw