Methyl (2E)-2-cyano-3-(2,2-difluoro-1,3-benzodioxol-4-yl)prop-2-enoate

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Principles of method if other than guideline:

Instead a centrifuged sludge a settled sludge was used. Due tos the poor solubility of the test substance at test concentrations, no stock solution was prepared. The test substance was given directly into the medium.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

methyl (2E)-2-cyano-3-(2,2-difluoro-1,3-benzodioxol-4-yl)prop-2-enoate

EC Number:

700-526-7

Cas Number:

1333488-95-1

Molecular formula:

C12H7F2NO4

IUPAC Name:

methyl (2E)-2-cyano-3-(2,2-difluoro-1,3-benzodioxol-4-yl)prop-2-enoate

Test solutions

Details on test solutions:

20.0, 6.5, 2.6, 0.67 and 0.34 mg of the test substance were weighed and added to the test medium. The volume was adjusted to 200 ml with water and aerated for 3 hours .
Resulting test substance concentrations: 100.0, 32.5, 13.0, 3.35.0 and 1.7 mg/l

Test organisms

Test organisms (species):

activated sludge

Details on inoculum:

Activated sludge collected from the sewage treatment plant of CH-4153 Reinach on 11/02/91. The preparation was carried out according to the method described in the guideline. The sludge was separated from the aqueous layer by settling instead of centrifugation.

Study design

Water media type:

freshwater

Total exposure duration:

3 h

Test conditions

Test temperature:

20 +/- 2°C

Nominal and measured concentrations:

nominal:
100.0, 32.5, 13.0, 3.35.0 and 1.7 mg/l

Reference substance (positive control):

yes

Remarks:

3,5-Dichlorophenole

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Any other information on results incl. tables

Respiration inhibition

Sample	Conc. [mg/l]	Consumption rate [mg/l/h]	Inhibition [%]
Blank 1	0	50.94	3
Blank 2	0	54.61	-3
Reference 1	32	9.18	83
Reference 2	10	36.29	31
Reference 3	3.2	50.59	4
Test conc. 1	100.6	53.41	-1
Test conc. 2	32.5	54.55	-3
Test conc. 3	13	52.75	0
Test conc. 4	3.35	52.98	0
Test conc. 5	1.7	51.00	3