Methyl (2E)-2-cyano-3-(2,2-difluoro-1,3-benzodioxol-4-yl)prop-2-enoate

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

yes

Principles of method if other than guideline:

Mean fish length is 64 mm (46-75 mm) based on 10 fishes. No stock solution was prepared because of the very low solubility of the test substance both in water and solvents. Test concentrations not maintained within the limits given by the guideline.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

methyl (2E)-2-cyano-3-(2,2-difluoro-1,3-benzodioxol-4-yl)prop-2-enoate

EC Number:

700-526-7

Cas Number:

1333488-95-1

Molecular formula:

C12H7F2NO4

IUPAC Name:

methyl (2E)-2-cyano-3-(2,2-difluoro-1,3-benzodioxol-4-yl)prop-2-enoate

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Composite samples (about 150 ml in duplicate) of each test concentration were drawn by mixing identical volumes of the test solutions taken from the approximate center of the test vessels. They were taken inunediately before the exposure and after 96 hours exposure and kept at - 18°C to - 22°C until analysis.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow Trout (Salmo gairdneri)
- Source: P. Höhler / CH-4314 Zeiningen
- Length at study initiation (mean and range): 64 mm (46-75 mm) based on 10 fishes
- Weight at study initiation (mean and range): 2.21 g (0.8 - 3.54g) based on 10 fishes
- Feeding during test: none

ACCLIMATION
- Acclimation period: 13 days

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

169 mg CaCO3/l

Test temperature:

13 - 17°C

pH:

8.1 - 8.4

Dissolved oxygen:

97-106 %

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

13.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Concentration nominal [mg/l]	Mortalities Number of dead fish 24 h	Mortalities Number of dead fish 48 h	Mortalities Number of dead fish 72 h	Mortalities Number of dead fish 96 h
Blank	0	0	0	0
1.8	0	0	0	0
3.2	0	0	0	0
5.8	0	0	0	0
10	0	0	0	0
18	10	10	10	10

Mortalities (initial numbers of fish = 10/testconc)