Registration Dossier
Registration Dossier
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EC number: 700-526-7 | CAS number: 1333488-95-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- (1981)
- Deviations:
- yes
- Principles of method if other than guideline:
- The volume of the test solution was reduced from 3.0 l to 1.5 l. The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve.a better distribution in the medium. The test substance was added to the medium, homogenized with Polyoxyethylen-Sorbitan-Monooleate (TWEEN 80).
- GLP compliance:
- yes
Test material
- Reference substance name:
- methyl (2E)-2-cyano-3-(2,2-difluoro-1,3-benzodioxol-4-yl)prop-2-enoate
- EC Number:
- 700-526-7
- Cas Number:
- 1333488-95-1
- Molecular formula:
- C12H7F2NO4
- IUPAC Name:
- methyl (2E)-2-cyano-3-(2,2-difluoro-1,3-benzodioxol-4-yl)prop-2-enoate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Bacteria collected from activated sludge of the sewage treatment plant of CH-4153 Reinach. The preparation was carried out according to the method described in the guideline.
- Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 11.1 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20.9 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Vessels: 2 liter flasks equipped with gas inlet and magnetic stirrer
- Air: ca. 25 ml/min purified from carbon dioxide
- Test temperature: 22 +/- 2 °C
Results and discussion
% Degradationopen allclose all
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 30
- Sampling time:
- 28 d
- Remarks on result:
- other: result for 11.1 mg test substance/l
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 14
- Sampling time:
- 28 d
- Remarks on result:
- other: result for 20.9 mg test substance/l
- Details on results:
- 11.1 mg test substance/l corresponds to 30.84 mg CO2/1.5 liter
20.9 mg test substance/l corresponds to 58.34 mg CO2/1.5 liter
BOD5 / COD results
- Results with reference substance:
- The biodegradation after 28 d for the reference substance was calculated to be 87 %
Applicant's summary and conclusion
- Interpretation of results:
- under test conditions no biodegradation observed
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