Reaction product of 3,9-dibromobenzanthrone condensed with 2 equivalents of 1-aminoanthraquinone, subsequently further condensed under oxidative conditions

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 June 1993 to 5 January 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

no analytical determination

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

yes

Remarks:

no analytical determination

GLP compliance:

yes

Analytical monitoring:

no

Details on sampling:

All test medium samples for the analytical determinations were taken after mixing of the test solutions from the approximate center of the test vessels and kept at -18°C to -25 °C until analyses.

Vehicle:

no

Details on test solutions:

0.5 g test substance were mixed with 200 ml test medium and fill up to 500 ml with test medium. The stock solution was homogenized for ten minutes in the supersonics bath.

Test organisms (species):

Daphnia magna

Details on test organisms:

The test organism was the species Daphnia magna. The daphnids were bred in the laboratories of the Textile Dyes Division, Product Ecology TF under standardized conditions. For the test, animals with age of less than 24 hours were used. The quality of the daphnids will be checked at least every 3 months by determining the EC50 values (24 h) for potassium dichromate (range: 0.8 -1.5 mg/l).
For each concentration, 20 Daphnia magna were used.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

No post exposure observation period specified.

Hardness:

136 mg/l CaCO3

Test temperature:

21.2 - 21.9°C

pH:

7.9 - 8.1

Dissolved oxygen:

97%

Salinity:

No data

Conductivity:

No data

Nominal and measured concentrations:

Based on a pretest, the test concentrations were chosen as 58 mg/l, 100 mg/l and 180 mg/l nominal

Details on test conditions:

48 hours at 21°C, 16 hours light / 8 hours darkThe daphnids were exposed to the test article in 250 ml beakers containing 100 - 200 ml test medium with 10 animals per beaker. All tests are running in duplicate. During exposure the animals were kept at 21 ± 1°C with 16 hours illumination.Test evaluations were done according to the guidelines by visual assessment of mobility of the daphnids after 24 and 48 hours.The EC50 was evaluated directly from the test concentrations.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

The test substance is not soluble in water and organic solvents. Therefore, the concentrations of the test substance could not be determined.
In the control and in all test concentrations no immobilization of the daphnids was observed after 48 hours. The EC 0 value is > 180 mg/l. The EC 50 and EC 100 values are clearly higher than 180 mg/l nominal.

Results with reference substance (positive control):

Reference substance not tested in this study.

Reported statistics and error estimates:

Not specified

 

Conc. of Test substance (mg/l nominal)	No. of animals	No. of responses		% immobility
		24 h	48 h	24 h	48 h
Blank	10 10	0 0	0 0	0	0
58	10 10	0 0	0 0	0	0
100	10 10	0 0	0 0	0	0
180	10 10	0 0	0 0	0	0

 

The EC values are evaluated as follows: (mg/l)

Time	EC 0	EC 50	EC 100
After 24 h After 48 h	≥180 ≥180	>180 >180	>180 >180

FAT-45’019/E is considered to be practically non-toxic to daphnids

Validity criteria fulfilled:

yes

Conclusions:

EC50 >180 mg/l nominal
The test material was practically non toxic to Daphnia magna.

Executive summary:

The acute toxicity of the test material to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adapted 4 April 84, and EEC Directive 84/449, L 251, Part C 2.

The test was performed in compliance with the Good Laboratory Practice Regulations.

 

The test substance is not soluble in water and organic solvents. Therefore, the concentration of the test substance could not be determined.

 

The nominal concentrations tested were 58 / 100 and 180 mg test article/l, and a control without any additions.

 

In the control and in all test concentrations no immobilization of the daphnids was observed after 48 hours. The EC 0 value is > 180 mg/l. The EC 50 and EC 100 values are clearly higher than 180 mg/l nominal.

Therefore, the EC 50 could not be calculated.

 

Results :

EC0 >180mg/l nominal

EC50 > 180mg/l nominal

EC100 >180mg/l nominal

 

Conclusion: The test material is practically non toxic to Daphnia magna.

Description of key information

Key value determined in a GLP accredited laboratory study performed in accordance with OECD Guideline 202 and EU Method C 2.

According to this test, the substance is practically non-toxic to Daphnia magna

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

180 mg/L

Additional information

The acute toxicity of the test material to Daphnia magna was determined in a GLP study performed in accordance with OECD Guideline 202 and EU Method C.2. The nominal concentrations tested were 58 mg/L, 100 mg/L and 180 mg/L, and a control without any additions. The control and all test concentrations did not cause any immobilization of the daphnids after 48 hours. The EC0 value is > 180 mg/L. The EC50 and EC100 values are clearly higher than 180 mg/L nominal. The test substance is not soluble in water and organic solvents therefore measured concentrations could not be determined. It is concluded that the substance is practically non-toxic to Daphnia magna.