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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2018
Report date:
2018

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
(E)-anethole
EC Number:
224-052-0
EC Name:
(E)-anethole
Cas Number:
4180-23-8
Molecular formula:
C10H12O
IUPAC Name:
1-methoxy-4-prop-1-en-1-ylbenzene

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Species: Daphnia magna
Variety: STRAUS
Strain: Berlin
Sex: female
Age: between 0 and 24 hours

Study design

Water media type:
other: Deionised water with an enrichment of certain minerals was used in the test
Total exposure duration:
21 d
Remarks on exposure duration:
Loading: 80 mL/animal, animals were kept individually Replicates: 10 replicates/treatment Blank Control : dilution water Lighting: 1000Lux Aeration: none Feeding: 0.15-0.20 mg org. carbon/animal/day, the amount of C is based on photometric measurement

Test conditions

Hardness:
Blank Control: old 267; new 267
22 mg/L: old 267-356; new:267-356
Test temperature:
20 ± 2 °C
pH:
Blank Control: old 7.6 - 8.0 ; new 7.8 - 8.1
22 mg/L: old 7.8 - 8.1; new:7.9 - 8.1
Dissolved oxygen:
Blank Control: old 8.0 - 8.7 ; new 8.7 - 9.4
22 mg/L: old 8.2 - 8.5; new: 8.7 - 9.3
Salinity:
Deionised water with an enrichment of certain minerals was used in the test.
Nominal and measured concentrations:
1 / 2.2 / 4.6 / 10 / 22 mg/L nominal

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
ca. 1.05 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Key result
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
ca. 2.44 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Key result
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
ca. 2.81 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

One valid experiment was performed using 5 concentrations ranging from 1.0 to 22 mg/L nominal concentration. Ten daphnia per concentration were exposed individually to the test item and the control for 21 days.

The offspring of the animals was counted three times a week. Decrease in reproduction was observed in the two highest treatments, ranging from 26 % to 98 %, compared to the control.The highest concentration showed also clear mortality (80 %).

The content of the test item in the test solutions was determined using GC/FID determination. The analysis of the test item concentrations in the treatments before and after exposition showed an acceptable stability of the test item during exposition. The geometric means of the measured concentrationsin the fresh prepared and the old solutionswere calculated. Their total mean was used for the evaluation of the biological results.

The test item was present in the solutions on day 2, but in very low concentrations, because of adsorption or ingestion onto the algae cells and daphnids. Therefore, one additional replicate per concentration without daphnids and algae were prepared for the analytical measurement. The geometric mean of day 0-2 was not used for evaluation of biological results.

 

The following results were determined for the test itemSynthetic Anethole 21/22:

 

 

NOEC = 1.05 mg/L
LOEC = 2.44 mg/L
EC50= 2.81 mg/L