Registration Dossier
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EC number: 216-676-7 | CAS number: 1638-86-4
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
In an 96 h acute toxicity test with Zebrafish (Brachydanio rerio), the median lethal concentrations (based on the nominal concentrations) were LC 50 (96 h) is > 215.0 and < 464.0 mg/L. Mortality occurred first at the concentration 464 mg/L. Other substance-related effects were the symptoms apathy, narcotic-like state, swimming near water surface, swimming near the bottom.
The no observed effect concentration NOEC (96 h) was 215 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 215 mg/L
Additional information
In a 96-h acute toxicity study, Zebrafish (Brachydanio rerio HAM. and BUCH.) were exposed to the test substance at nominal concentrations of 0, 46.4, 100, 215, 464 and 1000 mg/L under static conditions. The 96-h LC50 was determined to be between 215 and 464 mg/L. 100 % mortality (LC100) was observed at the concentraion 1000 mg/L. The NOEC value, based on mortality, was 215 mg/L. Sublethal effects of apathy, narcotic-like state and swimming near the water surface or the bottom were observed in the groups exposed to 464 mg/L of the test substance. Based on the results of this study, the test substance can be classified as not toxic to fish in accordance with the classification system of the EU CLP.
This toxicity study is classified as acceptable and satisfies the guideline requirement for the acute toxicity study on the zebra fish (Brachydanio rerio).
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