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EC number: 248-654-8
CAS number: 27776-01-8
% Formazan production (Tissue 1)
% Formazan production (Tissue 2)
% Formazan production (Tissue 3)
% Formazan production Mean
Three tissues of the human skin mode! EpiDerm™were treated with
(phenylethyl)benzene for 60 minutes.
30 µL of the liquid test item (using a nylon mesh) were applied to
each tissue and spread to match the tissue size (0.63 cm2; as
indicated by supplier).
DPBS-buffer was used as negative control, 5% SDS-solution was used
as positive control.
After treatment with the negative control, the absorbance values
were within the required acceptability criterion of 1.0 < mean OD < 2.5.
The positive control showed clear irritating effects. Variation within
tissues was acceptable (<18 %).
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